College of Art and Science, Northeast Agricultural University, Harbin 150030, China.
College of Pharmacy, Harbin Medical University, Harbin 150030, China.
Carbohydr Polym. 2021 May 15;260:117796. doi: 10.1016/j.carbpol.2021.117796. Epub 2021 Feb 13.
The polysaccharide (DRP) was gained from dandelion roots by ultrasonic-assisted enzymatic extraction (UAEE) followed by two-step column purification. Then selenylation of DRP has been accomplished by HNO-NaSeO method. sDRP-1 and sDRP-2 with the selenium content of 170 ± 1.13 and 710 ± 4.00 μg/g were prepared for further structural characterization and bioactivity determination. DRP, sDRP-1, and sDRP-2 were composed of the same monosaccharides in different molar ratios, and the molecular weights of DRP, sDRP-1 and sDRP-2 were 8700, 7900, and 5600 Da, respectively. Fourier transform infrared (FT-IR) spectra confirmed that DRP, sDRP-1, and sDRP-2 possessed similar functional groups. The results of Congo red test, X-ray diffraction (XRD) and scanning electron microscopy (SEM) showed that DRP, sDRP-1, and sDRP-2 had no three helix structure, did not form single crystal, and all belonged to amorphous morphology. sDRP-1 and sDRP-2 possessed greater antioxidant activities in vitro than the native polysaccharide DRP. At the same time, the selenized polysaccharides showed better immunomodulatory ability and could be used as new-type immunoenhancer. The present conclusions provided theoretical basis for the new application of dandelion polysaccharides and the development of dandelion resources.
多糖（DRP）通过超声辅助酶解（UAEE）从蒲公英根中获得，然后通过两步柱纯化。然后通过 HNO-NaSeO 法对 DRP 进行硒化。硒化后的 DRP 分别制备得到 sDRP-1 和 sDRP-2，其硒含量分别为 170±1.13μg/g 和 710±4.00μg/g，用于进一步的结构表征和生物活性测定。DRP、sDRP-1 和 sDRP-2 由相同的单糖组成，但摩尔比不同，DRP、sDRP-1 和 sDRP-2 的分子量分别为 8700、7900 和 5600Da。傅里叶变换红外（FT-IR）光谱证实 DRP、sDRP-1 和 sDRP-2 具有相似的功能基团。刚果红试验、X 射线衍射（XRD）和扫描电子显微镜（SEM）的结果表明，DRP、sDRP-1 和 sDRP-2 没有三螺旋结构，不形成单晶，均属于无定形形态。sDRP-1 和 sDRP-2 在体外具有比天然多糖 DRP 更强的抗氧化活性。同时，硒化多糖具有更好的免疫调节能力，可以作为新型免疫增强剂。本研究为蒲公英多糖的新应用和蒲公英资源的开发提供了理论基础。
