环状 RNA circBACH1（hsa_circ_0061395）作为 miR-656-3p 的海绵体，通过增加 SERBP1 表达促进肝细胞癌进展。
CircRNA circBACH1 (hsa_circ_0061395) serves as a miR-656-3p sponge to facilitate hepatocellular carcinoma progression through increasing SERBP1 expression.
机构信息
Department of Hepatobiliary Surgery, The First Affiliated Hospital of Nanchang University, NanChang, JiangXi, 330006, China.
Department of Hepatobiliary Surgery, The First Affiliated Hospital of Nanchang University, NanChang, JiangXi, 330006, China.
出版信息
Biochem Biophys Res Commun. 2021 Jun 4;556:1-8. doi: 10.1016/j.bbrc.2021.03.136. Epub 2021 Apr 5.
Hsa_circ_0061395(circBACH1) and SERBP1(SERPINE1 mRNA binding protein 1) have been reported to play a carcinogenic role in HCC.In this study, circBACH1, microRNA(miR)-656-3p, and SERBP1 expression levels with quantitative real-time polymerase chain reaction (qRT-PCR) in HCC tissue specimens and cells.The protein levels of SERBP1, E-Cadherin, vimentin, and N-Cadherin were detected with western blotting.Cell proliferation, migration, invasion, and apoptosis were determined with CCK-8, colony formation, transwell, and flow cytometry assays.The targeting relatio-nship between circBACH1 or SERBP1 and miR-656-3p was verified by dual-lucifer- ase reporter assay.The role of circBACH1 was validated by xenograft assay.CircBAC- H1 and SERBP1 were upregulated in HCC tissues and cells.Both circBACH1 and SERBP1 knockdown constrained proliferation, migration, invasion, and EMT(epithel-ial-mesenchymal transition), and facilitated apoptosis of HCC cells in vitro.Knockdo-wn of circBACH1 reduced HCC growth in vivo. SERBP1 overexpression partially neutralized the repressive effect of circBACH1 silencing on malignant behaviors of HCC cells.CircBACH1 sponged miR-656-3p to elevate SERBP1 expression, thereby accelerating the progression of HCC.The research provided a new evidence to support the role of circBACH1 in HCC.
hsa_circ_0061395（circbach1）和 serpBP1（丝氨酸蛋白酶抑制剂 E1 mRNA 结合蛋白 1）已被报道在 HCC 中发挥致癌作用。在本研究中，通过定量实时聚合酶链反应（qRT-PCR）检测 HCC 组织标本和细胞中 circbach1、微小 RNA（miR）-656-3p 和 serpBP1 的表达水平。用 Western blot 检测 serpBP1、E-钙黏蛋白、波形蛋白和 N-钙黏蛋白的蛋白水平。用 CCK-8、集落形成、transwell 和流式细胞术检测细胞增殖、迁移、侵袭和凋亡。通过双荧光素酶报告基因检测验证 circbach1 或 serpBP1 与 miR-656-3p 的靶向关系。通过异种移植实验验证 circbach1 的作用。circbach1 和 serpBP1 在 HCC 组织和细胞中上调。circbach1 和 serpBP1 的敲低均抑制 HCC 细胞的增殖、迁移、侵袭和 EMT（上皮-间充质转化），并促进 HCC 细胞凋亡。circbach1 的敲低减少了 HCC 在体内的生长。serpBP1 的过表达部分中和了 circbach1 沉默对 HCC 细胞恶性行为的抑制作用。circbach1 吸附 miR-656-3p 以升高 serpBP1 的表达，从而加速 HCC 的进展。该研究提供了新的证据支持 circbach1 在 HCC 中的作用。
Zotero 插件