Department of Gastroenterology, 117971The First Hospital of Jilin University, Changchun City, Jilin Province, China.
Department of Hematology, 117971The First Hospital of Jilin University, Changchun City, Jilin Province, China.
Cell Transplant. 2020 Jan-Dec;29:963689720963948. doi: 10.1177/0963689720963948.
Circular RNAs (circRNAs) could sponge micro-RNAs (miRNAs) to regulate tumor progression of hepatocellular carcinoma (HCC). Hsa_circ_104566 contributes to papillary thyroid carcinoma progression. However, the tumorigenic mechanism of hsa_circ_104566 on HCC remains enigmatic. The role of hsa_circ_104566 on HCC was therefore evaluated in this study. First, the high expression of hsa_circ_104566 was found in HCC tissues, which was significantly associated with poor prognosis in HCC patients. Second, Hsa_circ_104566 promoted HCC progression by decreasing apoptosis and E-cadherin, while increasing cell viability, proliferation, migration, invasion, and N-cadherin. On the other hand, HCC progression was suppressed by knockdown of hsa_circ_104566. Hsa_circ_104566 could target miR-338-3p, and its expression was negatively correlated with miR-338-3p in HCC patients. Moreover, miR-338-3p suppressed protein expression of Forkhead box protein 1 (FOXP1) and had a negative correlation with FOXP1 in HCC patients. Functional assay further indicated that the promotion of HCC progression by hsa_circ_104566 was reversed by miR-338-3p, and miR-338-3p inhibitor could counteract the effect of hsa_circ_104566 knockdown on the suppression of HCC progression. assay indicated that hsa_circ_104566 knockdown suppressed HCC tumor growth and metastasis. In conclusion, hsa_circ_104566 sponged miR-338-3p to promote HCC progression, providing a potential therapeutic target for cancer intervention.
环状 RNA（circRNAs）可以通过海绵吸附 microRNA（miRNA）来调节肝细胞癌（HCC）的肿瘤进展。Hsa_circ_104566 促进甲状腺乳头状癌的进展。然而，hsa_circ_104566 在 HCC 中的致瘤机制仍然难以捉摸。因此，本研究评估了 hsa_circ_104566 在 HCC 中的作用。首先，发现 hsa_circ_104566 在 HCC 组织中高表达，与 HCC 患者的预后不良显著相关。其次，hsa_circ_104566 通过降低细胞凋亡和 E-钙黏蛋白，同时增加细胞活力、增殖、迁移、侵袭和 N-钙黏蛋白来促进 HCC 进展。另一方面，hsa_circ_104566 的敲低抑制了 HCC 的进展。hsa_circ_104566 可以靶向 miR-338-3p，并且其表达与 HCC 患者中 miR-338-3p 的表达呈负相关。此外，miR-338-3p 抑制叉头框蛋白 1（FOXP1）的蛋白表达，并且在 HCC 患者中与 FOXP1 呈负相关。功能测定进一步表明，hsa_circ_104566 通过 miR-338-3p 促进 HCC 进展，而 miR-338-3p 抑制剂可以抵消 hsa_circ_104566 敲低对抑制 HCC 进展的作用。体内测定表明，hsa_circ_104566 敲低抑制 HCC 肿瘤生长和转移。总之，hsa_circ_104566 通过海绵吸附 miR-338-3p 促进 HCC 进展，为癌症干预提供了一个潜在的治疗靶点。
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