Kramer R H, Rosen S D, McDonald K A
Department of Anatomy, School of Medicine, University of California, San Francisco 94143.
Cell Tissue Res. 1988 May;252(2):367-75. doi: 10.1007/BF00214379.
Lymph nodes contain an extensive array of extracellular matrix fibers frequently referred to as "reticular fibers" because of their reticular pattern and positive reaction with silver stains. These fibers are known to contain primarily type-III collagen. In the present study, frozen and plastic-embedded sections of mouse and human lymph nodes were subjected to immunostaining with a panel of monospecific antibodies directed against type-IV collagen, type-III collagen, laminin, entactin, and heparan sulfate proteoglycan. Immunofluorescent staining revealed that, in addition to being uniformly stained with antibodies to type-III collagen, these fibers also stained positively with antibodies to type-IV collagen and to other basement-membrane-specific components. Furthermore, the basement-membrane-specific antibodies stained the outer surface of individual fibers. These same type-III collagen-rich fibers were distinct from blood vascular basement membranes since they did not react with antibodies to factor VIII-related antigen, an endothelial-cell-specific marker. The role of these basement-membrane-specific components associated with the reticular fibers of lymphoid tissue is unknown. However, it is possible that the ligands promote attachment of reticular fibroblasts as well as macrophages and lymphocytes to the extracellular matrix fibers.
淋巴结含有大量细胞外基质纤维，因其呈网状结构且对银染呈阳性反应，常被称为“网状纤维”。已知这些纤维主要包含III型胶原蛋白。在本研究中，对小鼠和人类淋巴结的冰冻切片及塑料包埋切片，用一组针对IV型胶原蛋白、III型胶原蛋白、层粘连蛋白、巢蛋白和硫酸乙酰肝素蛋白聚糖的单特异性抗体进行免疫染色。免疫荧光染色显示，除了被III型胶原蛋白抗体均匀染色外，这些纤维还用IV型胶原蛋白抗体和其他基底膜特异性成分抗体呈阳性染色。此外，基底膜特异性抗体对单个纤维的外表面进行了染色。这些富含III型胶原蛋白的纤维与血管基底膜不同，因为它们不与VIII因子相关抗原（一种内皮细胞特异性标志物）的抗体发生反应。这些与淋巴组织网状纤维相关的基底膜特异性成分的作用尚不清楚。然而，这些配体有可能促进网状成纤维细胞以及巨噬细胞和淋巴细胞附着于细胞外基质纤维。
