Lee C, Chen L B
Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
Cell. 1988 Jul 1;54(1):37-46. doi: 10.1016/0092-8674(88)90177-8.
Endoplasmic reticulum (ER) was studied by fluorescence microscopy of living CV-1 cells treated with the fluorescent carbocyanine dye DiOC6(3). Using video recording and image processing techniques, several distinct forms of highly localized movements of ER were documented, categorized, and analyzed in terms of mechanism and structural implications. These include tubule branching, ring closure, and sliding. These localized movements have been observed to generate the basic elements of ER: linear tubules, polygonal reticulum, and triple junctions. We propose that as such they act as the mechanism for constructing the polygonal lattice of interconnected membrane tubules that constitutes ER. The nature of these movements suggests possible involvement of the cytoskeleton, and, in view of the close correlations in the distributions of ER and microtubules, and the accompanying paper (Dabora and Sheetz), it is possible that microtubules may play a role in generating ER motility and in constructing and maintaining the ER network in living cells.
利用荧光碳菁染料DiOC6(3)处理活的CV-1细胞，通过荧光显微镜对内质网（ER）进行了研究。运用视频记录和图像处理技术，记录、分类并分析了内质网几种不同形式的高度局部化运动，并从机制和结构影响方面进行了探讨。这些运动包括小管分支、环闭合和滑动。已观察到这些局部化运动产生了内质网的基本组成部分：线性小管、多边形网状结构和三联体。我们认为，它们以此作为构建由相互连接的膜小管组成的多边形晶格的机制，而这种多边形晶格构成了内质网。这些运动的性质表明细胞骨架可能参与其中，并且鉴于内质网和微管分布的紧密相关性以及随附论文（达博拉和希茨），微管有可能在活细胞中产生内质网运动性以及构建和维持内质网网络方面发挥作用。
