Mossman K L
Exp Hematol. 1977 Nov;5(6):487-98.
The statistical distribution of colony counts in the spleen colony assay was determined in C57BL/6 and C3H mice under varying conditions of the assay procedure. Assay mice were given syngeneic bone marrow cells from normal or irradiated donors. The mice were injected intravenously with marrow cells either immediately or 3 days after irradiation. Eight days after engraftment, spleen colonies were counted and the statistical distribution of colony counts was determined using a mean to variance ratio criterion. Analysis of the data indicated that the statistical distribution of colony counts was different in the two strains of mice. In addition, delaying time of injection of mice with bone marrow cells or injecting bone marrow cells form irradiated donors also changed the statistical distribution. The significance of this work lies in the fact that modification of the usual assay procedure can alter the statistical distribution of colony counts, thus changing the statistical accuracy of the assay. Modification in the assay procedure, such that an increase occurs in the variance of the distribution of colony counts, must be compensated for by increasing the number of spleens counted in order to maintain a constant level of statistical accuracy in experiments.
在脾集落测定实验的不同条件下，测定了C57BL/6和C3H小鼠脾集落计数的统计分布。实验小鼠接受来自正常或经辐照供体的同基因骨髓细胞。小鼠在辐照后立即或3天后静脉注射骨髓细胞。移植8天后，计数脾集落，并使用均值与方差比标准确定集落计数的统计分布。数据分析表明，两种品系小鼠的集落计数统计分布不同。此外，延迟给小鼠注射骨髓细胞的时间或注射来自经辐照供体的骨髓细胞也会改变统计分布。这项工作的意义在于，改变通常的测定程序可以改变集落计数的统计分布，从而改变测定的统计准确性。为了在实验中保持恒定的统计准确性水平，必须通过增加计数的脾脏数量来补偿测定程序的改变，这种改变会导致集落计数分布的方差增加。
