Department of Physiology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Katowice, Poland.
Department of Instrumental Analysis, Faculty of Pharmaceutical Sciences in Sosnowiec Medical University of Silesia, Katowice, Poland.
Sci Rep. 2021 Apr 8;11(1):7774. doi: 10.1038/s41598-021-87293-w.
We studied the long-term effect of ileal transposition (IT) metabolic surgery on the hepatokines: retinol-binding protein-4 (RBP4), α-2-HS-glycoprotein (aHSG/fetuin-A), and fibroblast growth factor 21 (FGF21), C-reactive protein (CRP) plasma levels, glucose metabolism, body weight, liver histology, as well as total lipids concentration in muscle, liver, and fat tissue of obese Zucker (Crl:ZUC(ORL)-Lepr) rats. 14 adult males were randomly submitted either to IT or SHAM (control) surgery. Pre-operative hepatokines plasma levels were not significantly different in rats submitted to IT or SHAM protocol. Three months after the procedures the plasma levels of RBP4, aHSG, FGF21, and CRP were significantly lower in IT-operated animals when compared to SHAM-operated group. Three and 12 weeks after the IT and SHAM surgery, the AUC were significantly lower than AUC before the surgery. HOMA-IR was lower in rats after IT surgery in comparison to the SHAM-operated rats. Muscle and liver total lipids concentration was reduced after the IT procedure when compared to pre-IT conditions. IT had a significant reductive impact on the body weight in comparison to SHAM surgery in the 4th, 6th, 8th, and 10th week after the surgery. We conclude that IT reduces hepatokines' plasma concentrations, muscle and liver total lipids concentration but not the inflammatory processes in the liver of Zucker (Crl:ZUC(ORL)-Lepr) rats.
我们研究了回肠转位（IT）代谢手术后对肝系数：视黄醇结合蛋白 4（RBP4）、α-2-HS-糖蛋白（aHSG/胎球蛋白-A）和纤维母细胞生长因子 21（FGF21）、C-反应蛋白（CRP）血浆水平、葡萄糖代谢、体重、肝组织学以及肌肉、肝脏和脂肪组织中总脂质浓度的长期影响肥胖 Zucker（Crl：ZUC（ORL）-Lepr）大鼠。14 只成年雄性大鼠随机接受 IT 或 SHAM（对照）手术。接受 IT 或 SHAM 方案的大鼠术前肝系数血浆水平无显著差异。手术后 3 个月，与 SHAM 手术组相比，IT 手术组大鼠的 RBP4、aHSG、FGF21 和 CRP 血浆水平显著降低。IT 和 SHAM 手术后 3 周和 12 周，AUC 明显低于手术前。与 SHAM 手术组相比，IT 手术后大鼠的 HOMA-IR 较低。与 IT 前相比，IT 手术后大鼠的肌肉和肝脏总脂质浓度降低。与 SHAM 手术相比，IT 手术后大鼠的体重在第 4、6、8 和 10 周时明显降低。我们得出结论，IT 降低了 Zucker（Crl：ZUC（ORL）-Lepr）大鼠的肝系数血浆浓度、肌肉和肝脏总脂质浓度，但不影响肝脏的炎症过程。
