Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentiev Ave., Novosibirsk, Russia, 630090.
Department of Natural Sciences, Novosibirsk State University, 2 Pirogova Str., Novosibirsk, Russia, 630090.
Sci Rep. 2021 Apr 8;11(1):7789. doi: 10.1038/s41598-021-87446-x.
Helicobacter pylori causes a wide range of human diseases including cancer. Carcinogenic foodborne trematodes Opisthorchis viverrini, Clonorchis sinensis, and O. felineus might promote transmission and spread of H. pylori infection in the definitive mammalian host, which in turn might contribute to the liver fluke-associated malignancy. Our objectives were to find out whether liver flukes O. felineus, O. viverrini, and C. sinensis are carriers of Helicobacter pylori and to determine whether H. pylori is present in feces, bile, and stomach samples from the experimentally infected hamsters. We found that liver flukes are not reservoirs of H. pylori. Nevertheless, the prevalence of H. pylori and the H. pylori ureA gene copy number were significantly elevated after the infection. Overall, although the liver flukes O. felineus, C. sinensis, and O. viverrini are not reservoirs of H. pylori, the infection with the liver flukes significantly modifies the biliary and gut microbiota by increasing H. pylori abundance. This may be a feature of any liver fluke pathogenesis that have not previously been taken into account. Our findings appear to be novel in terms of comparative assessment of the host microbiota and Helicobacter abundance during epidemiologically important liver fluke infections.
幽门螺杆菌可引起广泛的人类疾病，包括癌症。致癌性食源性吸虫华支睾吸虫、肝片形吸虫和猫后睾吸虫可能促进幽门螺杆菌在终末哺乳动物宿主中的传播和扩散，而这反过来又可能导致与肝吸虫相关的恶性肿瘤。我们的目的是确定肝吸虫猫后睾吸虫、华支睾吸虫和肝片形吸虫是否携带幽门螺杆菌，并确定幽门螺杆菌是否存在于实验感染的仓鼠的粪便、胆汁和胃样本中。我们发现肝吸虫不是幽门螺杆菌的储存库。然而，感染后，幽门螺杆菌的流行率和幽门螺杆菌 ureA 基因拷贝数显著升高。总的来说，尽管肝吸虫猫后睾吸虫、肝片形吸虫和华支睾吸虫不是幽门螺杆菌的储存库，但肝吸虫的感染通过增加幽门螺杆菌的丰度显著改变了胆汁和肠道微生物群。这可能是任何以前未被考虑过的肝吸虫发病机制的一个特征。就流行病学上重要的肝吸虫感染期间宿主微生物群和幽门螺杆菌丰度的比较评估而言，我们的发现似乎是新颖的。
