Masisi Kabo, Masamba Riach, Lashani Keletso, Li Chunyang, Kwape Tebogo E, Gaobotse Goabaone
Department of Biological Sciences and Biotechnology, Faculty of Sciences, Botswana International University of Science and Technology, Palapye, Botswana.
Department of Nutrition and Health, Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, China.
J Pharmacopuncture. 2021 Mar 31;24(1):24-31. doi: 10.3831/KPI.2021.24.1.24.
Accumulation of cellular reactive oxygen species (ROS) leads to oxidative stress. Increased production of ROS, such as superoxide anion, or a deficiency in their clearance by antioxidant defences, mediates cellular pathology. fruits are a source of bioactive compounds and have notable antioxidant activity. Although the antioxidant capacity of has been studied, there is very limited evidence that links the antioxidant activities of and to the inhibition of free radical formation associated with damage in biological systems.
This study evaluated the protective effects of and extracts against free radical-induced oxidative stress and the resulting cytotoxicity effect using HeLa cells. Antioxidant properties determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and total phenolic content (TPC) assays showed significantly higher (p < 0.05) antioxidant activity in (ethanol extract) than (water extract) and (ethanol and water extracts).
Using 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide or MTT assay, cytotoxicity results showed that extracts of and were less toxic to HeLa cells at tested concentrations compared to the untreated control. This confirmed the low toxicity of these edible fruits at the tested concentrations in HeLa cells. Furthermore, hydrogen peroxide (HO)-induced cell loss was effectively reduced by pre-incubating HeLa cells with and extracts, with (ethanol extract) revealing better protection.
The effect was speculated to be associated with the higher antioxidant activity of (ethanol extract). Additional studies will warrant confirmation of the mechanism of action of such effects.
细胞活性氧（ROS）的积累会导致氧化应激。超氧阴离子等ROS生成增加，或抗氧化防御对其清除能力不足，会介导细胞病变。水果是生物活性化合物的来源，具有显著的抗氧化活性。尽管已对[未提及的物质]的抗氧化能力进行了研究，但将[未提及的物质]和[未提及的物质]的抗氧化活性与抑制生物系统中与损伤相关的自由基形成联系起来的证据非常有限。
本研究使用HeLa细胞评估了[未提及的物质]和[未提及的物质]提取物对自由基诱导的氧化应激及由此产生的细胞毒性作用的保护效果。使用2,2 - 二苯基 - 1 - 苦基肼（DPPH）和总酚含量（TPC）测定法确定的抗氧化特性表明，[未提及的物质]（乙醇提取物）的抗氧化活性显著高于[未提及的物质]（水提取物）和[未提及的物质]（乙醇和水提取物）（p < 0.05）。
使用3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐或MTT测定法，细胞毒性结果显示，与未处理的对照相比，在测试浓度下[未提及的物质]和[未提及的物质]的提取物对HeLa细胞的毒性较小。这证实了这些可食用水果在测试浓度下对HeLa细胞的低毒性。此外，通过将HeLa细胞与[未提及的物质]和[未提及的物质]提取物预孵育，过氧化氢（HO）诱导的细胞损失得到有效减少，[未提及的物质]（乙醇提取物）显示出更好的保护作用。
推测该作用与[未提及的物质]（乙醇提取物）较高的抗氧化活性有关。进一步的研究将有必要确认这种作用的作用机制。
