Lamola Stella Makgabo, Dzoyem Jean Paul, Botha Francien, van Wyk Candice
Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, Pretoria, South Africa.
Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Afr Health Sci. 2017 Sep;17(3):790-796. doi: 10.4314/ahs.v17i3.22.
Bacterial infections of the gastrointestinal tract (GIT) cause vomiting, diarrhoea and even systemic disease. There is a need for the development of natural products into alternative and safer medicines.
This study evaluated the anti-microbial activity of extracts prepared from berries, leaves, bark and roots of the edible plant .
The anti-bacterial activity was evaluated by the broth microdilution method. Anti-oxidant activity of the most active extracts was performed by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The cytotoxicity of the extracts was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
The acetone extracts of the leaves and roots showed the best activity with MIC values as low as 0.03 mg/mL against and and 0.07 mg/mL against and . Quantitative analysis of the scavenging ability showed that acetone extracts exhibited good free radical scavenging activity in a dose-dependent manner. The berries extract had the highest LC (lowest toxicity) of 551.68 68 µg/mL.
Acetone extract of leaves and roots of contain anti-microbial and anti-oxidant compounds and could therefore be used as a natural product with little toxicity to host cells.
胃肠道细菌感染会导致呕吐、腹泻甚至全身性疾病。有必要将天然产物开发成替代且更安全的药物。
本研究评估了从可食用植物的浆果、叶子、树皮和根中制备的提取物的抗菌活性。
采用肉汤微量稀释法评估抗菌活性。通过2,2-二苯基-1-苦基肼（DPPH）法测定最具活性提取物的抗氧化活性。使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法测定提取物的细胞毒性。
叶子和根的丙酮提取物表现出最佳活性，对[具体细菌1]和[具体细菌2]的最低抑菌浓度（MIC）值低至0.03 mg/mL，对[具体细菌3]和[具体细菌4]的MIC值为0.07 mg/mL。清除能力的定量分析表明，丙酮提取物以剂量依赖方式表现出良好的自由基清除活性。浆果提取物的半数致死浓度（LC，最低毒性）最高，为551.68±68 µg/mL。
[植物名称]叶子和根的丙酮提取物含有抗菌和抗氧化化合物，因此可作为对宿主细胞毒性较小的天然产物使用。
