Lu Yan, Xiao Yi, Yin Ming-Zhu, Zhou Xing-Chen, Wu Li-Sha, Chen Wang-Qing, Luo Yan, Kuang Ye-Hong, Zhu Wu
Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China.
Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, China.
Front Med (Lausanne). 2021 Mar 22;7:560579. doi: 10.3389/fmed.2020.560579. eCollection 2020.
To explore the possible mechanism of improving the imiquimod (IMQ)-induced psoriasis-like inflammation by using polyethylene glycol (PEG) ointment. We evaluated the appearance of psoriasis lesions by Psoriasis Area and Severity Index (PASI), observed the epidermal proliferation by histopathological staining and immunohistochemical staining, and explored the key molecules and signaling pathways of improving psoriasis-like inflammation treated with PEG ointment by RNA sequencing. Finally, we verified the expression of inflammatory cells and inflammatory factors by flow cytometry, immunohistochemical staining, and Q-PCR. PEG ointment could improve the appearance of psoriasis lesions and the epidermis thickness of psoriasis mouse, inhibit the proliferation of keratinocytes, and down-regulate the relative mRNA levels of IL-23, IL-22, IL-6, IL-17C, IL-17F, S100A7, S100A8, S100A9, CXCL1, CXCL2, and IL-1β in the skin lesions of psoriasis mouse by down-regulating the numbers of myeloid-derived suppressor cells (MDSCs) and T helper 17 (Th17) cells. PEG ointment could improve the IMQ-induced psoriasis-like inflammation by down-regulating the functions of Th17 cells and MDSCs.
为探究聚乙二醇（PEG）软膏改善咪喹莫特（IMQ）诱导的银屑病样炎症的可能机制。我们通过银屑病面积和严重程度指数（PASI）评估银屑病皮损外观，通过组织病理学染色和免疫组织化学染色观察表皮增殖，并通过RNA测序探索PEG软膏治疗改善银屑病样炎症的关键分子和信号通路。最后，我们通过流式细胞术、免疫组织化学染色和Q-PCR验证炎症细胞和炎症因子的表达。PEG软膏可改善银屑病小鼠的皮损外观和表皮厚度，抑制角质形成细胞增殖，并通过下调髓源性抑制细胞（MDSCs）和辅助性T细胞17（Th17）细胞数量，下调银屑病小鼠皮肤病变中IL-23、IL-22、IL-6、IL-17C、IL-17F、S100A7、S100A8、S100A9、CXCL1、CXCL2和IL-1β的相对mRNA水平。PEG软膏可通过下调Th17细胞和MDSCs的功能来改善IMQ诱导的银屑病样炎症。
