Tefiani Ikram, Lahbib Seddiki Sidi Mohammed, Yassine Mahdad Moustafa
Department of Antifungal Antibiotic, Physico-Chemical Synthesis, and Biological Activity, University of Tlemcen, Tlemcen, Algeria.
University Center of Naâma, Naâma, Algeria.
Curr Med Mycol. 2020 Sep;6(3):27-32. doi: 10.18502/cmm.6.3.4499.
is an important microorganism in the normal flora of a healthy subject; however, it has an expedient pathogenic character that induces hydrolytic virulence. Regarding this, the present study aimed to find an in vitro alternative that could reduce the virulence of this yeast.
For the purpose of the study, the effect of amphotericin B (AmB) combined with the extract of (E1) or (E2) was evaluated against the hydrolytic activities of esterase, protease, and phospholipase. This effect was determined by calculating the minimum inhibitory concentration (MIC), used to adjust the extract/AmB mixtures in culture media.
The evaluated Pz values, which corresponded to the different enzymatic activities, showed a decrease in the hydrolytic activities of strains after the addition of E1/AmB and E2/AmB combinations at descending concentrations (lower than the obtained MICs).
Based on the findings, it would be possible to reduce the pathogenesis of this species without destabilizing the balance of the flora.
是健康受试者正常菌群中的一种重要微生物；然而，它具有诱导水解毒力的便利致病特性。关于此，本研究旨在找到一种体外替代方法，以降低这种酵母的毒力。
为了本研究目的，评估了两性霉素B（AmB）与 提取物（E1）或 提取物（E2）联合使用对酯酶、蛋白酶和磷脂酶水解活性的影响。通过计算最低抑菌浓度（MIC）来确定这种效果，该浓度用于在培养基中调整提取物/AmB混合物。
对应于不同酶活性的评估Pz值显示，在添加浓度逐渐降低（低于获得的MIC）的E1/AmB和E2/AmB组合后， 菌株的水解活性降低。
基于这些发现，有可能在不破坏菌群平衡的情况下降低该物种的致病性。
