Falahi Sara, Salari Farhad, Rezaiemanesh Alireza, Mortazavi Seyed Hamidreza, Koohyanizadeh Farzaneh, Lotfi Ramin, Gorgin Karaji Ali
Student Research Committee, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Immunol Res. 2021 Apr;69(2):189-195. doi: 10.1007/s12026-021-09189-1. Epub 2021 Apr 8.
Interleukin-12 (IL-12) is a heterodimeric cytokine encoded by two separate genes, IL12A and IL12B, which may play a regulatory role in allergen-induced inflammation through CD4 T-cell subsets polarization. The aim of this study was to investigate the association of single-nucleotide polymorphisms (SNPs) in the IL12B gene with susceptibility to allergic rhinitis (AR). We performed a case-control study including 130 AR patients and 130 healthy controls to evaluate the possible association between IL12B gene SNPs (rs3212227, rs6887695) and the risk of AR using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Our results showed no significant association between IL12B rs3212227 A > C polymorphism with AR. In contrast, the GC genotype of rs6887695 G > C was associated with susceptibility to AR in comparison with the GG genotype (p = 0.049, OR = 1.684, 95% CI: 1.002-2.83). We also observed a statistically significant difference in the additive model (GC versus GG + CC, p = 0.03, OR = 1.705, 95% CI: 1.040-2.794) for SNPs rs6887695. Furthermore, haplotypes analysis demonstrated that C-C haplotype was associated with an increased risk of AR (p = 0.01, OR = 1.845, 95% CI: 1.114-3.057). Our findings suggest that IL12B rs6887695 polymorphism may be a potential biomarker for susceptibility to AR in an Iranian population.
白细胞介素-12（IL-12）是一种由两个独立基因IL12A和IL12B编码的异二聚体细胞因子，其可能通过CD4 T细胞亚群极化在变应原诱导的炎症中发挥调节作用。本研究的目的是调查IL12B基因单核苷酸多态性（SNP）与变应性鼻炎（AR）易感性之间的关联。我们进行了一项病例对照研究，纳入130例AR患者和130例健康对照，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法评估IL12B基因SNP（rs3212227、rs6887695）与AR风险之间的可能关联。我们的结果显示，IL12B rs3212227 A>C多态性与AR之间无显著关联。相比之下，与GG基因型相比，rs6887695 G>C的GC基因型与AR易感性相关（p = 0.049，OR = 1.684，95%CI：1.002-2.83）。我们还观察到，对于SNP rs6887695，在加性模型（GC与GG + CC相比，p = 0.03，OR = 1.705，95%CI：1.040-2.794）中存在统计学显著差异。此外，单倍型分析表明，C-C单倍型与AR风险增加相关（p = 0.01，OR = 1.845，95%CI：1.114-3.057）。我们的研究结果表明，IL12B rs6887695多态性可能是伊朗人群AR易感性的潜在生物标志物。
