Laboratory of Ecological Microbiology, Department of Microbiology, Faculty of Nature and Life Sciences, University of Bejaia, 06000, Bejaïa, Algeria.
Metabolic Integration and Cell Signaling Laboratory, Plant Physiology Section, Department Ciencias Agrarias Y del Medio Natural, Universitat Jaume I, 12071, Castelló de la Plana, Spain.
Curr Microbiol. 2021 Jun;78(6):2367-2379. doi: 10.1007/s00284-021-02483-z. Epub 2021 Apr 9.
Bacteria endophytes are living microorganisms that live inside plant tissues without visible harmful symptoms, providing a mutualistic interaction. In this study, different bacterial endophytic strains were isolated from different plants primed to live in an arid area, namely, the Sahara Desert. Up to 27 of these strains were selected based on their ability to inhibit Botrytis cinerea growth in dual-culture assay and by bacterial volatiles. The results presented in this study show the capacity of most of the bacterial strains to protect Solanum lycopersicum against the pathogenic fungus B. cinerea, under different experimental conditions. Five of these strains induced susceptibility in tomato plants and no callose accumulation upon fungal infection, pointing to callose deposition as a protective mechanism mediated by endophytic bacteria. Moreover, there was a significant correlation between the bacterial strains inducing callose and the level of protection against B. cinerea. On the other hand, hormone production by bacteria does not explain the relationship between protection and the differences between the phenotypic results obtained in vitro and those obtained in plant experiments. Induced resistance is highly specific in the inducer-plant-stress interaction.
植物内生细菌是生活在植物组织内的微生物，没有可见的有害症状，提供互利的相互作用。在这项研究中，从适应干旱地区（即撒哈拉沙漠）生活的不同植物中分离出不同的内生细菌菌株。根据它们在双培养试验中抑制灰葡萄孢生长的能力和细菌挥发物的能力，选择了多达 27 种这些菌株。本研究的结果表明，大多数细菌菌株具有在不同实验条件下保护番茄植物免受致病真菌灰葡萄孢侵害的能力。其中 5 种菌株诱导番茄植物易感性，真菌感染时不积累胼胝质，表明胼胝质沉积是内生细菌介导的保护机制。此外，诱导胼胝质的细菌菌株与对灰葡萄孢的保护水平之间存在显著相关性。另一方面，细菌产生激素并不能解释保护作用与体外获得的表型结果和植物实验结果之间的差异之间的关系。在诱导剂-植物-胁迫相互作用中，诱导抗性具有高度特异性。
