Gill V J, Williams D
Clinical Pathology Department, Clinical Center, National Institutes of Health, Bethesda, MD 20892.
Diagn Microbiol Infect Dis. 1988 Feb;9(2):119-21. doi: 10.1016/0732-8893(88)90105-8.
We evaluated the use of horse blood agar containing 0.0005% pyridoxal hydrochloride (HBAP) mainly for use as a primary plating medium for blood cultures, other sterile fluids, wounds, and abscesses in order to facilitate the isolation of nutritionally deficient streptococci (NDS). Our results showed that the addition of pyridoxal hydrochloride (pxl) permitted good growth of NDS within 24 h and did not inhibit or significantly alter the colony morphology of 34 different species of microorganisms and 296 isolates of beta hemolytic streptococci presumptively identified as Group A streptococci. The use of pxl-supplemented blood agar such as HBAP is recommended for primary plating as well as subculturing of blood cultures, and may also increase recovery of NDS from wounds and abscesses.
我们评估了含0.0005%盐酸吡哆醛的马血琼脂（HBAP）的用途，其主要用作血培养、其他无菌液体、伤口和脓肿的初次接种培养基，以便于分离营养缺陷型链球菌（NDS）。我们的结果表明，添加盐酸吡哆醛（pxl）可使NDS在24小时内良好生长，且不抑制或显著改变34种不同微生物及296株初步鉴定为A组链球菌的β溶血性链球菌的菌落形态。建议使用添加pxl的血琼脂如HBAP进行血培养的初次接种及传代培养，其还可能提高从伤口和脓肿中分离出NDS的几率。
