Reimer L G, Reller L B
J Clin Microbiol. 1981 Sep;14(3):329-32. doi: 10.1128/jcm.14.3.329-332.1981.
Nutritionally variant streptococci fail to grow on routine sheep blood agar plates. Moreover, these strains are a recognized cause of culture-negative endocarditis. We tested the ability of chocolate and brucella blood agars, sheep blood agar with a staphylococcal streak, sheep blood agar with 0.001% pyridoxal, and 10 commercial blood culture media from two manufactures to grow these bacteria. Of the original 25 strains tested, 16 were recovered on chocolate agar, 21 were recovered on brucella blood agar and 21 were recovered on sheep blood agar with a staphylococcal streak. Sheep blood agar with pyridoxal grew all 22 strains tested. Supplemented peptone, thioglycolate, and thiol broths grew all strains, but brain heart infusion and three tryptic soy broths supported five or fewer strains. The addition of 5 ml of human blood improved recovery to 100% in all media except tryptic soy broths. Unless supplemented wih pyridoxal, common laboratory agars were inadequate for recovering all strains of variant streptococci upon subculture of blood culture bottles. As used clinically, the blood culture media that we studied other than tryptic soy broths should reliably grow these bacteria.
营养变异链球菌无法在常规绵羊血琼脂平板上生长。此外，这些菌株是已知的培养阴性心内膜炎的病因。我们测试了巧克力琼脂、布鲁氏菌血琼脂、带有葡萄球菌划线的绵羊血琼脂、含有0.001%吡哆醛的绵羊血琼脂以及来自两家制造商的10种商业血培养培养基培养这些细菌的能力。在最初测试的25株菌株中，16株在巧克力琼脂上生长，21株在布鲁氏菌血琼脂上生长，21株在带有葡萄球菌划线的绵羊血琼脂上生长。含有吡哆醛的绵羊血琼脂上所有22株测试菌株均生长。补充蛋白胨、硫乙醇酸盐和硫醇肉汤可使所有菌株生长，但脑心浸液和三种胰蛋白胨大豆肉汤仅支持五种或更少菌株生长。除胰蛋白胨大豆肉汤外，在所有培养基中添加5毫升人血可使回收率提高到100%。除非补充吡哆醛，否则普通实验室琼脂在对血培养瓶进行传代培养时不足以培养出所有变异链球菌菌株。在临床应用中，我们研究的除胰蛋白胨大豆肉汤外的血培养培养基应该能够可靠地培养这些细菌。
