MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, P. R. China.
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117585, Singapore.
Angew Chem Int Ed Engl. 2021 Jun 25;60(27):15095-15100. doi: 10.1002/anie.202104163. Epub 2021 May 26.
Ferroptosis regulates cell death through reactive oxygen species (ROS)-associated lipid peroxide accumulation, which is expected to affect the structure and polarity of lipid droplets (LDs), but with no clear evidence. Herein, we report the first example of an LD/nucleus dual-targeted ratiometric fluorescent probe, CQPP, for monitoring polarity changes in the cellular microenvironment. Due to the donor-acceptor structure of CQPP, it offers ratiometric fluorescence emission and fluorescence lifetime signals that reflect polarity variations. Using nucleus imaging as a reference, CQPP was applied to report the increase in LD polarity and the homogenization of polarity between LDs and cytoplasm in the ferroptosis model. This LD/nucleus dual-targeted fluorescent probe shows the great potential of using fluorescence imaging to study ferroptosis and ferroptosis-related diseases.
铁死亡通过活性氧（ROS）相关的脂质过氧化物积累来调节细胞死亡，这预计会影响脂滴（LDs）的结构和极性，但目前尚无明确证据。在此，我们报道了首个 LD/核双靶比率荧光探针 CQPP 的实例，用于监测细胞微环境中极性的变化。由于 CQPP 的供体-受体结构，它提供了反映极性变化的比率荧光发射和荧光寿命信号。使用核成像作为参考，CQPP 被用于报告铁死亡模型中 LD 极性的增加和 LD 与细胞质之间极性的均匀化。这种 LD/核双靶荧光探针显示出使用荧光成像研究铁死亡和铁死亡相关疾病的巨大潜力。
