Zhai Baoping, Hu Wei, Zhai Shuyang, Liang Tianyu, Liu Zhihong
Department of Chemistry, Xinzhou Normal University, Xinzhou, Shanxi, 034000, China.
Department of Chemistry, Xinzhou Normal University, Xinzhou, Shanxi, 034000, China.
Talanta. 2023 May 1;256:124304. doi: 10.1016/j.talanta.2023.124304. Epub 2023 Feb 2.
As a novel pattern of regulated cell death (RCD), Ferroptosis is induced by lipid peroxide-dependent iron accumulation, which is associated with reactive oxygen species (ROS). Ferroptosis regulates cell death via ROS accumulation-related lipid peroxides accumulation, affecting the structure and polarity of lipid droplets (LDs). Compared with reactive fluorescent probes, environment-sensitive fluorescent probes allow for maximum preservation of the intracellular environment while monitoring metabolic activity in situ, resulting in more accurate monitoring results. In this study, a polarity-sensitive two-photon fluorescent probe with anchoring capacity in LDs, LIP-Pola, is reported and applied to monitor the polarity of LDs during cell Ferroptosis by in situ imaging analysis of cell Ferroptosis via LDs polarity changes. Additionally, Paclitaxel is shown to increase the Ferroptosis level from data of cells and tumor tissue sections, suggesting that Paclitaxel may deactivate tumor cells by regulating Ferroptosis.
作为一种新型的程序性细胞死亡（RCD）模式，铁死亡由脂质过氧化物依赖性铁积累诱导，这与活性氧（ROS）相关。铁死亡通过与ROS积累相关的脂质过氧化物积累来调节细胞死亡，影响脂滴（LDs）的结构和极性。与反应性荧光探针相比，环境敏感型荧光探针在原位监测代谢活性时能最大程度地保留细胞内环境，从而获得更准确的监测结果。在本研究中，报道了一种在脂滴中具有锚定能力的极性敏感双光子荧光探针LIP-Pola，并通过对细胞铁死亡过程中脂滴极性变化进行原位成像分析，将其应用于监测细胞铁死亡过程中脂滴的极性。此外，从细胞和肿瘤组织切片的数据显示，紫杉醇可提高铁死亡水平，这表明紫杉醇可能通过调节铁死亡来使肿瘤细胞失活。
