Royce P M, Barnes M J
Strangeways Research Laboratory, Worts' Causeway, Cambridge, England.
Connect Tissue Res. 1988;17(1):55-70. doi: 10.3109/03008208808992794.
Freshly isolated bone cells from embryonic chick calvariae were found to attach to films of native monomeric type I or II collagen, to gels of native type I collagen and, to a lesser extent, to air-dried films of these collagens, in a fibronectin-independent manner, and with no requirement for collagen synthesis. Adhesion to type III collagen was relatively poor. Films of native monomeric type I or II collagen were preferred to the corresponding air-dried films as substrata for proliferation. Cis-hydroxyproline inhibited growth upon both plastic and type I and type II collagenous substrata, but this effect could largely be overcome by providing bone cell-conditioned medium, suggesting the possibility of a requirement in the growth process for synthesis of a collagen other than type I, the major form in bone matrix.
研究发现，从胚胎鸡颅骨中新鲜分离出的骨细胞能够以不依赖纤连蛋白的方式，且无需胶原蛋白合成，附着于天然单体I型或II型胶原蛋白薄膜、天然I型胶原蛋白凝胶以及在较小程度上附着于这些胶原蛋白的风干薄膜。骨细胞对III型胶原蛋白的黏附相对较差。相较于相应的风干薄膜，天然单体I型或II型胶原蛋白薄膜更适合作为骨细胞增殖的底物。顺式羟脯氨酸抑制骨细胞在塑料以及I型和II型胶原底物上的生长，但通过提供骨细胞条件培养基，这种抑制作用在很大程度上可以被克服，这表明在生长过程中可能需要合成除I型胶原蛋白（骨基质中的主要形式）之外的其他胶原蛋白。
