Dickson I R, Walls J, Webb S
Department of Medicine, University of Cambridge Clinical School, Addenbrooke's Hospital, U.K.
Biochim Biophys Acta. 1989 Oct 9;1013(3):254-8. doi: 10.1016/0167-4889(89)90143-2.
The response of bone cells in organ culture to retinol and retinoic acid was studied. Both stimulated incorporation of [3H]thymidine into DNA by 16-day embryonic chick calvaria, but the time-course of the responses differed; the peak responses to retinol and retinoic acid occurred at about 18 h and 48 h, respectively. Although retinol inhibited chick bone collagen synthesis retinoic acid had no effect, but it did stimulate non-collagenous protein synthesis, whereas the effect on the latter of retinol was, if anything, inhibitory. When present with retinol, retinoic acid was able to attenuate the inhibitory effect of the former on chick bone collagen synthesis, but preincubation with retinoic acid had no such effect. In neonatal murine calvarial cultures, retinoic acid inhibited collagen synthesis selectively in the same manner as did retinol. The ability of chick osteoblasts to respond differently to retinol and retinoic acid suggests that both forms of the vitamin may have a role in bone formation and that their intracellular models of action may differ although the attenuation response indicates there may be some interaction between the two.
研究了器官培养中的骨细胞对视黄醇和视黄酸的反应。二者均刺激16日龄鸡胚颅骨将[3H]胸腺嘧啶核苷掺入DNA，但反应的时间进程不同；对视黄醇和视黄酸的峰值反应分别出现在约18小时和48小时。虽然视黄醇抑制鸡骨胶原蛋白合成，但视黄酸无此作用，不过它确实刺激了非胶原蛋白合成，而视黄醇对后者的作用即使有也是抑制性的。当与视黄醇同时存在时，视黄酸能够减弱前者对鸡骨胶原蛋白合成的抑制作用，但预先用视黄酸孵育则无此效果。在新生小鼠颅骨培养物中，视黄酸与视黄醇一样选择性地抑制胶原蛋白合成。鸡成骨细胞对视黄醇和视黄酸产生不同反应的能力表明，这两种形式的维生素可能在骨形成中都起作用，并且它们的细胞内作用模式可能不同，尽管减弱反应表明二者之间可能存在一些相互作用。
