Molecular, Cellular and Developmental Biology Department (MCD), Centre de Biologie Integrative (CBI), University of Toulouse, CNRS, UPS, Toulouse, France.
Nat Cell Biol. 2021 Apr;23(4):305-313. doi: 10.1038/s41556-021-00663-4. Epub 2021 Apr 9.
R-loops are non-B DNA structures with intriguing dual consequences for gene expression and genome stability. In addition to their recognized roles in triggering DNA double-strand breaks (DSBs), R-loops have recently been demonstrated to accumulate in cis to DSBs, especially those induced in transcriptionally active loci. In this Review, we discuss whether R-loops actively participate in DSB repair or are detrimental by-products that must be removed to avoid genome instability.
R 环是具有有趣的双重影响的非 B 型 DNA 结构，对基因表达和基因组稳定性都有影响。除了它们在触发 DNA 双链断裂 (DSB) 方面的公认作用外，最近还证明 R 环会在 cis 中积累到 DSB，尤其是在转录活跃的基因座中诱导产生的 DSB。在这篇综述中，我们讨论了 R 环是否积极参与 DSB 修复，或者它们是否是有害的副产物，必须清除这些副产物以避免基因组不稳定。
