Institute for Infectious Diseases, University of Bern, Bern, Switzerland; Unisanté, the Center for Primary Care and Public Health, Department of Occupational and Environmental Health (DSTE), University of Lausanne, Switzerland.
Institute for Infectious Diseases, University of Bern, Bern, Switzerland.
Sci Total Environ. 2021 Aug 15;782:146842. doi: 10.1016/j.scitotenv.2021.146842. Epub 2021 Mar 30.
Previous studies have suggested a significantly higher diversity in the nasal microbiota of pig farmers compared to people having no contact with farm animals. However, the fate of this nasal microbiota specificity after farmers stop being in contact with the pig farm environment is unknown. The aim of this study was to investigate the change in the nasal microbiota of pig-farmers after the change of occupation. METHODS: Anterior and posterior nasal swabs were collected from seven people during employment on pig farms, and again after a period of at least 50 days after leaving the pig farm. Illumina MiSeq sequencing of 16S rRNA was conducted to characterize the dynamics of the nasal microbiota. The microbiota of actively working pig farmers was compared to microbiota after they had stopped working (ex-pig-farmers) and to control groups (cow farmers and non-exposed individuals). RESULTS: Following a prolonged period without exposure to pigs, α-diversity of both anterior and posterior cavities dropped significantly. The composition of the microbiota of pig-farmers had a low inter-similarity with the non-exposed group while ex-pig-farmers were more similar to cow-farmers and the non-exposed group than to their own microbiota during pig farming.
先前的研究表明，与没有接触过农场动物的人相比，养猪农民的鼻腔微生物组多样性明显更高。然而，养猪农民停止接触养猪环境后，这种鼻腔微生物组特异性的命运尚不清楚。本研究旨在调查养猪农民在职业改变后鼻腔微生物组的变化。方法：在养猪场工作期间，从前鼻腔和后鼻腔采集了 7 个人的拭子，然后在离开养猪场至少 50 天后再次采集。通过 Illumina MiSeq 测序 16S rRNA 来描述鼻腔微生物组的动态变化。将活跃工作的养猪农民的微生物组与停止工作后的（前养猪农民）和对照组（奶牛农民和未暴露个体）的微生物组进行比较。结果：在长时间不接触猪后，前鼻腔和后鼻腔的α多样性均显著下降。养猪农民的微生物组与未暴露组的相似性较低，而前养猪农民的微生物组与奶牛农民和未暴露组的相似性高于其在养猪时的微生物组。
