Jiang Dun, Wu Shuai, Tan Mingtao, Wang Qing, Zheng Lin, Yan Shan-Chun
School of Forestry, Northeast Forestry University, Harbin 150040, PR China; Key Laboratory of Sustainable Forest Ecosystem Management-Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
School of Forestry, Northeast Forestry University, Harbin 150040, PR China; Key Laboratory of Sustainable Forest Ecosystem Management-Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
Pestic Biochem Physiol. 2021 May;174:104805. doi: 10.1016/j.pestbp.2021.104805. Epub 2021 Feb 25.
Hyphantria cunea is one of the most destructive invasive agricultural and forest pests worldwide. In order to better understand the adaptation mechanism of H. cunea larvae to secondary metabolites of their highly diversified host plants, the physiological function and detoxification ability of midgut, as well as the gut microbial community were investigated in H. cunea larvae fed with cinnamic acid-treated artificial diets. Our results showed that cinnamic acid treatment could not affect the growth and food utilization of H. cunea larvae, as evidenced by a non-significantly altered larval body weight and efficiency of conversion of ingested food. Evaluation of oxidative stress-related parameters (e.g. malondialdehyde and hydrogen peroxide) and midgut histopathology also clearly confirmed that cinnamic acid treatment caused no significant oxidative damage and pathological changes in the larval midgut. Variance analysis showed that cinnamic acid treatment significantly increased the content of non-enzymatic antioxidants (ascorbic acid and glutathione), the activity of antioxidant enzymes (superoxide dismutase and peroxidase) and detoxification enzyme (carboxylate esterase), as well as the abundance of several gut microbiota at the genus level (Hydrogenophaga and Acinetobacter) involved in the organic substance degradation in larval midgut. Further Pearson's correlation analysis revealed that these strongly altered gut microbiota at the genus level appeared to be significantly correlated with the detoxification and antioxidation parameters. These findings demonstrate the high adaptability of H. cunea larvae to cinnamic acid involves in detoxification, antioxidation and gut microbiota response, and indicate the existence of an extremely effective counter-defense mechanism for H. cunea larvae against the secondary metabolites of host plants.
美国白蛾是全球最具破坏力的入侵性农林害虫之一。为了更好地了解美国白蛾幼虫对其高度多样化寄主植物次生代谢产物的适应机制，本研究对取食肉桂酸处理人工饲料的美国白蛾幼虫的中肠生理功能、解毒能力以及肠道微生物群落进行了研究。结果表明，肉桂酸处理不影响美国白蛾幼虫的生长和食物利用，幼虫体重和摄入食物转化率无显著变化。氧化应激相关参数（如丙二醛和过氧化氢）评估及中肠组织病理学检查也明确证实，肉桂酸处理未对幼虫中肠造成显著氧化损伤和病理变化。方差分析显示，肉桂酸处理显著提高了非酶抗氧化剂（抗坏血酸和谷胱甘肽）含量、抗氧化酶（超氧化物歧化酶和过氧化物酶）及解毒酶（羧酸酯酶）活性，以及幼虫中肠参与有机物降解的几个属水平肠道微生物（嗜氢菌属和不动杆菌属）的丰度。进一步的Pearson相关性分析表明，这些在属水平上显著改变的肠道微生物似乎与解毒和抗氧化参数显著相关。这些发现表明，美国白蛾幼虫对肉桂酸具有高度适应性，涉及解毒、抗氧化和肠道微生物反应，并表明美国白蛾幼虫针对寄主植物次生代谢产物存在极其有效的防御机制。
