Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China; Biotech & Biomed Research Institute, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China.
Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China; Biotech & Biomed Research Institute, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China.
Int J Biol Macromol. 2020 Nov 1;162:609-617. doi: 10.1016/j.ijbiomac.2020.06.099. Epub 2020 Jun 13.
By combining DEAE-cellulose-52 and Sepharose-CL-6B chromatography, a heteropolysaccharide ECIP-1A with an average molecular weight of 24,336 Da was isolated from the hyphae of Eurotium cristatum. The main components of the monosaccharides of ECIP-1A were mannose, glucose and galactose, and their molar ratio is 4.003: 0.592: 4.58. Analysis of the immunomodulatory activity showed that ECIP-1A could significantly promote the proliferation of RAW264.7 cells and induce the production of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and nitric oxide (NO) by RAW264.7 cells. The results therefore suggested that ECIP-1A, as an immunomodulator, may have potential application prospects in the food and pharmaceutical industries.
通过 DEAE-纤维素-52 和 Sepharose-CL-6B 层析的组合，从青霉菌的菌丝体中分离出一种平均分子量为 24,336 Da 的杂多糖 ECIP-1A。ECIP-1A 的单糖的主要成分是甘露糖、葡萄糖和半乳糖，其摩尔比为 4.003:0.592:4.58。免疫调节活性分析表明，ECIP-1A 能显著促进 RAW264.7 细胞的增殖，并诱导 RAW264.7 细胞产生肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和一氧化氮（NO）。因此，ECIP-1A 作为一种免疫调节剂，可能在食品和制药行业具有潜在的应用前景。
