大麻素 2 受体激动剂 JWH-133 对大鼠卵巢缺血再灌注损伤的保护作用。
Activation of cannabinoid 2 receptors by JWH-133 protects against ovarian ischemia-reperfusion injury in rats.
机构信息
Department of Obstetrics and Gynecology, Faculty of Medicine, Yozgat Bozok University, Yozgat, Turkey.
Department of Histology-Embryology, Faculty of Medicine, Yozgat Bozok University, Yozgat, Turkey.
出版信息
Biotech Histochem. 2021 Jul;96(5):394-400. doi: 10.1080/10520295.2021.1905183. Epub 2021 Apr 12.
We investigated the efficacy of activated cannabinoid 2 receptors for alleviating ovarian ischemia-reperfusion injury in rats. Female Wistar albino rats were divided randomly into six groups: ischemia-reperfusion (IRG); ischemia-reperfusion + 0.2 mg/kg JWH-133 (JIRG1), ischemia-reperfusion + 1 mg/kg JWH-133 (JIRG2); ischemia-reperfusion + 5 mg/kg JWH-133 (JIRG3); solvent control, and sham control. Ovarian ischemia was established for 3 h followed by reperfusion for 3 h. Ovarian tissue was investigated using histology, immunohistochemistry and biochemistry. Administration of JWH-133 synthetic cannabinoid reduced nuclear factor kappa-B immunoreactivity as well as TUNEL positivity scores and malondialdehyde levels. These reductions were significant in all cases except for the malondialdehyde levels in the 1 mg/kg JWH-133 group. Activation of cannabinoid 2 receptors by JWH-133 reduced ovarian ischemia-reperfusion injury due to its antioxidant and anti-inflammatory effects.
我们研究了激活大麻素 2 受体对减轻大鼠卵巢缺血再灌注损伤的疗效。雌性 Wistar 白化大鼠随机分为六组：缺血再灌注（IRG）；缺血再灌注+0.2mg/kg JWH-133（JIRG1），缺血再灌注+1mg/kg JWH-133（JIRG2）；缺血再灌注+5mg/kg JWH-133（JIRG3）；溶剂对照和假对照。卵巢缺血 3 小时，再灌注 3 小时。采用组织学、免疫组织化学和生物化学方法研究卵巢组织。JWH-133 合成大麻素的给药减少了核因子 kappa-B 免疫反应性以及 TUNEL 阳性评分和丙二醛水平。除 1mg/kg JWH-133 组的丙二醛水平外，所有情况下的这些减少均具有统计学意义。JWH-133 通过激活大麻素 2 受体，通过其抗氧化和抗炎作用减轻卵巢缺血再灌注损伤。
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