School of Pharmacy, China Medical University, Shenyang, China.
Department of Pharmacy, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Crit Rev Anal Chem. 2022;52(7):1624-1643. doi: 10.1080/10408347.2021.1907173. Epub 2021 Apr 12.
Nucleotides, which are important low-molecular-weight compounds present in organisms, are precursors of nucleic acids and participate in various regulatory and metabolic functions. Sensitive and valid methods for monitoring and determining nucleotides and nucleosides in different samples are urgently required. Due to the presence of numerous endogenous interferences in complex matrices and the high polarity of the molecules of the phosphate moiety, the determination of nucleotide content is challenging. This review summarizes the pretreatment and analysis methods of nucleotides in different samples. Advanced pretreatment methods, including different microextraction methods, solid-phase extraction based on novel materials, QuEChERS, are clearly displayed, and continuous progress which has been made in LC, LC-MS/MS and capillary electrophoresis methods are discussed. Moreover, the strengths and weaknesses of different methods are discussed and compared. Highlight:Advanced pretreatment and detection methods of nucleotides were critically reviewed.Microextraction technology was one of the trends of nucleotides pretreatment in the future.Applications of novel materials and supercritical fluid were highlighted.The evolution and advance of HRMS analyzers were in detailed.
核苷酸是存在于生物体中的重要的低分子量化合物，是核酸的前体，并参与各种调节和代谢功能。因此，迫切需要能够监测和测定不同样品中核苷酸和核苷的灵敏有效的方法。由于在复杂基质中存在大量的内源性干扰物以及磷酸部分分子的高极性，因此核苷酸含量的测定具有挑战性。本综述总结了不同样品中核苷酸的预处理和分析方法。先进的预处理方法，包括不同的微萃取方法、基于新型材料的固相萃取、QuEChERS 等，都有明确的展示，并且讨论了在 LC、LC-MS/MS 和毛细管电泳方法方面取得的持续进展。此外，还讨论并比较了不同方法的优缺点。
批判性地综述了核苷酸的先进预处理和检测方法。
微萃取技术是核苷酸预处理的未来趋势之一。
强调了新型材料和超临界流体的应用。
详细介绍了 HRMS 分析仪的演变和进步。
