School of Pharmacy, China Medical University, Shenyang, China.
Department of Pharmacy, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Crit Rev Anal Chem. 2021;51(1):55-86. doi: 10.1080/10408347.2019.1676194. Epub 2019 Oct 24.
Cephalosporins, one of the classic β-lactam antibiotics, has been widely concerned by global population as the most commonly used broad-spectrum antibiotic. Serious health hazards are possible to be posed by quality control problems caused by its unstable structure, as well as food and environmental pollution introduced by improper use. Given the reasons above, the sensitive and valid methods for monitor and determination of cephalosporins in different matrices are urgently required. This review aims to provide a comprehensive overview on the current status of the pretreatment and analysis methods of cephalosporins in bulk drug, pharmaceutics, animal-derived foodstuffs including eggs, milk, meat, environment samples, and biological samples. Pretreatment methods including simple steps (protein precipitation, centrifugation, filtration), liquid-liquid extraction, solid phase extraction, QuECHERS, and detection methods covering LC, LC-MS/MS, voltammetric sensor, capillary electrophoresis spectroscopy, biological methods from January 2005 to October 2018 are updated, elaborated and compared here. Moreover, advanced materials and prospects for development are discussed. HighlightsDetermination of cephalosporins in different newly found matrix are represented.Comparisons between different mass analyzers and progress in HRMS methods are in detailed.Optimization of experimental conditions are discussed.Newly emerged eco-friendly methods are introduced.
头孢菌素类抗生素是经典的β-内酰胺类抗生素之一，因其结构不稳定，以及使用不当导致食品和环境污染，成为全球人群最常用的广谱抗生素，可能存在严重的健康危害。鉴于上述原因，迫切需要敏感有效的方法来监测和测定不同基质中的头孢菌素类抗生素。本综述旨在全面概述 2005 年 1 月至 2018 年 10 月间，头孢菌素类抗生素在原料药、制剂、动物源性食品（包括鸡蛋、牛奶、肉类）、环境样品和生物样品中的预处理和分析方法的最新研究进展。文中更新、阐述和比较了包括简单步骤（蛋白质沉淀、离心、过滤）、液液萃取、固相萃取、QuECHERS 在内的预处理方法，以及涵盖 LC、LC-MS/MS、伏安传感器、毛细管电泳光谱法、生物方法等检测方法。此外，还讨论了先进材料和发展前景。
要点
代表了不同新基质中头孢菌素类抗生素的测定。
详细比较了不同质量分析器和 HRMS 方法的进展。
讨论了实验条件的优化。
介绍了新出现的环保方法。
