Han Lu, Li Dandan, Hang Yanwen, Zong Xiaojuan, Lv Jiashun, Bai Xiaolu, Lu Yi, Zhang Peng, Zhou Meiling, Wu Zhaodi, Hu Wei
Department of Cardiology, Minhang Hospital, Fudan University, Shanghai, China.
Front Genet. 2021 Mar 24;12:632164. doi: 10.3389/fgene.2021.632164. eCollection 2021.
Circular RNAs (circRNAs) are novel non-coding RNAs, which show abnormal expression in several diseases, such as atherosclerosis (AS). The purpose of the present study was to reveal the association between hsa_circ_0004543 and AS. In the present study, hsa_circ_0004543 was overexpressed in human umbilical vein endothelial cells (HUVECs) induced by oxidized low-density lipoprotein (oxLDL). Inhibition of hsa_circ_0004543 expression facilitated the proliferation, migration, and invasion of HUVECs and significantly reduced their apoptotic rate following treatment with oxLDL. Furthermore, silencing of hsa_circ_0004543 activated the PI3K/AKT/NOS3 pathway in oxLDL-induced HUVECs. Collectively, these results demonstrated that hsa_circ_0004543 may play a vital role in the development of AS and affect the proliferation of HUVECs, providing a potential target for treating endothelial cell damage in AS.
环状RNA（circRNAs）是一类新型的非编码RNA，其在包括动脉粥样硬化（AS）在内的多种疾病中呈现异常表达。本研究旨在揭示hsa_circ_0004543与AS之间的关联。在本研究中，hsa_circ_0004543在氧化型低密度脂蛋白（oxLDL）诱导的人脐静脉内皮细胞（HUVECs）中过表达。抑制hsa_circ_0004543的表达促进了HUVECs的增殖、迁移和侵袭，并显著降低了oxLDL处理后它们的凋亡率。此外，沉默hsa_circ_0004543激活了oxLDL诱导的HUVECs中的PI3K/AKT/NOS3通路。总体而言，这些结果表明hsa_circ_0004543可能在AS的发展中起重要作用，并影响HUVECs的增殖，为治疗AS中的内皮细胞损伤提供了一个潜在靶点。
