环状 RNA hsa_circ_0003575 调控 oxLDL 诱导的血管内皮细胞增殖和血管生成。
Circular RNA hsa_circ_0003575 regulates oxLDL induced vascular endothelial cells proliferation and angiogenesis.
机构信息
Department of Geratology, 2nd Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
Department of Cardiology, 2nd Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
出版信息
Biomed Pharmacother. 2017 Nov;95:1514-1519. doi: 10.1016/j.biopha.2017.09.064. Epub 2017 Sep 21.
Circular RNAs (circRNAs) are a novel class of RNAs generated from back-splicing and characterized by covalently closed continuous loops. Recently, circRNAs have recently shown large regulation on cardiovascular system, including atherosclerosis. The present study aims to investigate the circRNA expression profile and identify their roles on vascular endothelial cells induced by oxLDL. Human circRNA microarray analysis revealed that total 943 differently expressed circRNAs were screened with 2 fold change. Hsa_circ_0003575 was validated to be significantly up-regulated in oxLDL induced HUVECs. Loss-of-function experiments indicated that hsa_circ_0003575 silencing promoted the proliferation and angiogenesis ability of HUVECs. Bioinformatics online programs predicted the potential circRNA-miRNA-mRNA network for hsa_circ_0003575. In summary, circRNA microarray analysis reveals the expression profiles of HUVECs and verifies the role of hsa_circ_0003575 on HUVECs, providing a therapeutic strategy for vascular endothelial cell injury of atherosclerosis.
环状 RNA（circRNAs）是一类通过反向剪接产生的新型 RNA，其特征是共价闭合的连续环。最近，circRNAs 对心血管系统，包括动脉粥样硬化，有很大的调控作用。本研究旨在探讨 oxLDL 诱导的血管内皮细胞中 circRNA 的表达谱，并鉴定其作用。人环状 RNA 微阵列分析显示，用 2 倍变化筛选出总共有 943 个差异表达的 circRNAs。Hsa_circ_0003575 在 oxLDL 诱导的 HUVECs 中被验证为显著上调。功能丧失实验表明，hsa_circ_0003575 沉默促进了 HUVECs 的增殖和血管生成能力。生物信息学在线程序预测了 hsa_circ_0003575 的潜在 circRNA-miRNA-mRNA 网络。总之，circRNA 微阵列分析揭示了 HUVECs 的表达谱，并验证了 hsa_circ_0003575 在 HUVECs 中的作用，为动脉粥样硬化血管内皮细胞损伤提供了一种治疗策略。
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