Leippe M, Renwrantz L
Zoologisches Institut, Universität Hamburg, Federal Republic of Germany.
Dev Comp Immunol. 1988 Spring;12(2):297-308. doi: 10.1016/0145-305x(88)90006-7.
Hemolymph of Mytilus edulis is known to contain cytotoxic hemocytes and a serum agglutinin. This study describes the occurrence of strong hemolytic properties as well as agglutinating activity of a hemocyte extract against different types of erythrocytes. The hemolysin purified by preparative disc-electrophoresis and subjected to PAGE reveals one band which is also visible in the electropherogram of the extract or serum, respectively. Likewise, the serum agglutinin corresponds to another distinct fraction of extract and serum. Both, hemolytic and agglutinating molecules are detectable in the supernatant of a short-term hemocyte culture indicating that these substances are actively secreted by Mytilus blood cells.
已知紫贻贝的血淋巴含有细胞毒性血细胞和血清凝集素。本研究描述了血细胞提取物对不同类型红细胞具有很强的溶血特性以及凝集活性。通过制备性圆盘电泳纯化并进行聚丙烯酰胺凝胶电泳的溶血素显示出一条带，该带分别在提取物或血清的电泳图谱中也可见。同样，血清凝集素对应于提取物和血清的另一个不同组分。在短期血细胞培养物的上清液中可检测到溶血和凝集分子，这表明这些物质是由紫贻贝血细胞主动分泌的。
