Yoshino T P, Tuan T L
Dev Comp Immunol. 1985 Summer;9(3):515-22. doi: 10.1016/0145-305x(85)90014-x.
Serum-free hemocytes from the clam, Corbicula fluminea, are cytolytic to mammalian erythrocytes (RBCs) as demonstrated in a hemoglobin release assay. The reaction is hemocyte dose- and temperature-dependent, and is mediated by the release of soluble hemolytic factors from clam cells. Initiation of hemocyte secretory activity does not appear to require contact with target RBCs. The chemical nature of the secreted lysin(s) has not been determined; however, PAGE analysis of hemocyte extracts (lysates) reveals the presence of at least five peaks of lytic activity. This activity is sensitive to heat and proteolytic enzyme treatment, and can be removed by absorption with fixed, homologous RBCs. Thus it is likely that the secreted hemocyte lysin is a heat-labile protein which exerts its hemolytic activity by binding directly to target RBC membrane constituents.
如血红蛋白释放试验所示，河蚬无血清血细胞对哺乳动物红细胞（RBC）具有细胞溶解作用。该反应具有血细胞剂量和温度依赖性，由蛤细胞释放可溶性溶血因子介导。血细胞分泌活性的启动似乎不需要与靶红细胞接触。所分泌溶素的化学性质尚未确定；然而，血细胞提取物（裂解物）的PAGE分析显示至少有五个溶血活性峰。这种活性对热和蛋白酶处理敏感，并且可以通过与固定的同源红细胞吸附而去除。因此，所分泌的血细胞溶素可能是一种热不稳定蛋白，它通过直接结合靶红细胞膜成分发挥其溶血活性。
