Hughes T K, Smith E M, Leung M K, Stefano G B
Department of Microbiology, University of Texas Medical Branch, Galveston 77550.
Ann N Y Acad Sci. 1992 Apr 15;650:74-80. doi: 10.1111/j.1749-6632.1992.tb49098.x.
Mytilus edulis, a marine bivalve mollusc, contains hemocytes that share certain properties with cells of the human monocyte-macrophage lineage. Because of this and previously reported similar responses to opioids between Mytilus hemocytes and human granulocytes, we determined whether Mytilus might also possess monokines or an immune monokine-like network. We chose to study two monokines, interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF). We found that Mytilus hemocytes respond to recombinant human IL-1 and TNF in a fashion similar to human granulocytes. Furthermore, we show that as in the human system, it appears that IL-1 mediates at least part of its effects through immunoreactive (ir) TNF in Mytilus. Finally, we show that Mytilus hemolymph contains ir IL-1 and TNF. To our knowledge, this is the first demonstration of these substances in this system.
紫贻贝是一种海洋双壳贝类软体动物，其血细胞与人类单核细胞-巨噬细胞谱系的细胞具有某些共同特性。鉴于此，以及之前报道的紫贻贝血细胞与人类粒细胞对阿片类药物的类似反应，我们确定紫贻贝是否也可能拥有单核因子或免疫单核因子样网络。我们选择研究两种单核因子，即白细胞介素-1（IL-1）和肿瘤坏死因子-α（TNF）。我们发现紫贻贝血细胞对重组人IL-1和TNF的反应方式与人类粒细胞相似。此外，我们表明，与人类系统一样，在紫贻贝中，IL-1似乎至少部分通过免疫反应性（ir）TNF介导其作用。最后，我们表明紫贻贝血淋巴中含有ir IL-1和TNF。据我们所知，这是在该系统中首次证明这些物质的存在。
