Shimazu H, Shinzawa H, Togahi H, Wakabayashi H, Yamada N, Nakamura T, Takahashi T, Ishikawa M
Second Department of Internal Medicine, Yamagata University School of Medicine, Japan.
Gastroenterol Jpn. 1988 Apr;23(2):124-8. doi: 10.1007/BF02799023.
Computerized morphometrical measurements were made of liver cells and their nuclei taken from livers of healthy persons and from patients with acute hepatitis (AH), chronic hepatitis (CH), and liver cirrhosis (LC). Liver biopsies were performed on 127 people (24 convalescing from acute hepatitis, 20 with chronic inactive hepatitis (CIH), 24 with chronic active hepatitis (CAH), 38 with liver cirrhosis (LC) and 21 who were healthy controls (HC]. The specimens were stained with hematoxylin-eosin, then light microscopic pictures were obtained. The original cells were magnified 1000 times. The sizes of the cells and of their nuclei were calculated using a computer. Correlative variation was noted in cell and nuclear sizes resulting in an consistently constant nucleus-cytoplasm ratio. Average sizes of both cells and nuclei were maximal in livers with LC, followed in decreasing order by those in livers with CAH, CIH, HC and convalescent AH. The smallest cells and nuclei were found in convalescent AH cases. It might be possible to some degree to obtain gross impressions of the histopathological type of the liver lesion by measuring the sizes of cells and nuclei of the liver.
对取自健康人以及患有急性肝炎（AH）、慢性肝炎（CH）和肝硬化（LC）患者肝脏的肝细胞及其细胞核进行了计算机形态测量。对127人进行了肝活检（24例急性肝炎康复者、20例慢性非活动性肝炎（CIH）患者、24例慢性活动性肝炎（CAH）患者、38例肝硬化（LC）患者和21例健康对照者（HC））。标本用苏木精-伊红染色，然后获取光学显微镜图像。原始细胞放大1000倍。使用计算机计算细胞及其细胞核的大小。观察到细胞和细胞核大小的相关变化，导致核质比始终恒定。细胞和细胞核的平均大小在肝硬化肝脏中最大，其次按降序排列为患有CAH、CIH、HC的肝脏以及急性肝炎康复者的肝脏。在急性肝炎康复者病例中发现最小的细胞和细胞核。通过测量肝脏细胞和细胞核的大小，在一定程度上有可能获得肝脏病变组织病理学类型的大致印象。
