一种荆豆烷型三萜皂苷，来自的 sum 比洛斯苷 A 及其对季节性流感病毒 H3N2 的强大免疫调节活性。

A hederagenin-type triterpene saponin, sumbulianoside a from and its potent immunomodulatory activity against seasonal flu virus H3N2.

机构信息

Faculty of Science, Department of Chemistry, Ege University, Bornova, Turkey.

Faculty of Engineering, Department of Bioengineering, Ege University, Bornova, Turkey.

出版信息

Nat Prod Res. 2022 May;36(10):2495-2503. doi: 10.1080/14786419.2021.1910691. Epub 2021 Apr 11.

DOI:10.1080/14786419.2021.1910691

PMID:33843360

Abstract

A new hederagenin-type triterpene saponin; hederagenin 28-O--D-galactopyranosyl-(1→6)--D-glucopyranosyl ester named sumbulianoside A (), together with twelve known saponins were isolated from the -butanol extract of (Caprifoliaceae) from which, one known saponin, dipsacus saponin A () was isolated, for the first time from species. The structures of the isolated compounds were elucidated by 1 D and 2 D NMR and HRESIMS analyses. Cytotoxic activities were investigated on A549, Hela, PANC1, SHSY5Y cells and non-cancerous cell HEK293 by MTT method and immunomodulatory activities were evaluated against activated H3N2 seasonal virus in whole blood by measuring IL-4, IFN-, IL-1 cytokine level with ELISA. According to the cytotoxicity results, compounds and did not possess significant cytotoxicity, while only compound induced significant IL-4 production (** <.001) against H3N2 showing a possible Th2 response and antibody production. All compounds had no effect on cytokine release ( > 0.5).

摘要

一种新的羽扇豆烷型三萜皂苷；羽扇豆烷 28-O--D-半乳糖吡喃基-(1→6)--D-吡喃葡萄糖苷酯命名为 sumbulianoside A ()，与十二种已知皂苷一起从毛蕊花属（忍冬科）的正丁醇提取物中分离出来，其中一种已知皂苷，毛蕊花皂苷 A () 是首次从毛蕊花属植物中分离出来的。通过 1D 和 2D NMR 和 HRESIMS 分析阐明了分离化合物的结构。通过 MTT 法在 A549、Hela、PANC1、SHSY5Y 细胞和非癌细胞 HEK293 上研究了细胞毒性活性，并通过 ELISA 测量 IL-4、IFN-、IL-1 细胞因子水平评估了对激活的 H3N2 季节性病毒的免疫调节活性。根据细胞毒性结果，化合物和没有表现出显著的细胞毒性，而只有化合物对 H3N2 表现出显著的 IL-4 产生（** <.001），显示出可能的 Th2 反应和抗体产生。所有化合物对细胞因子释放均无影响（ > 0.5）。