Jin Cheng-Long, Wang Sheng-Lin, Wang Shuang, Zhang Ya-Nan, Xia Wei-Guang, Zhang Chang, Huang Xue-Bing, Li Kai-Chao, Zheng Chun-Tian, Chen Wei
State Key Laboratory of Swine and Poultry Breeding Industry/Institute of Animal Science, Guangdong Academy of Agricultural Sciences/Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs/ Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, PR China.
State Key Laboratory of Swine and Poultry Breeding Industry/Institute of Animal Science, Guangdong Academy of Agricultural Sciences/Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs/ Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, PR China.
Poult Sci. 2025 Jan;104(1):104551. doi: 10.1016/j.psj.2024.104551. Epub 2024 Nov 22.
The ovary is the main controller of female fertility, unfortunately, its onset of aging processes was earlier than other organs. Our previous studies showed calcium (Ca) deficiency reduced ovarian weight and declined numbers of dominant follicles in an avian model. However, whether Ca provided a functional role in follicle development of aged avian, and its further mechanism was still unknown. In this study, fifty180-day-old and fifty 700-day-old female Longyan ducks were divided into the young group and the aged group to illustrate the differences of Ca signaling and further mechanisms. We found the poor productive performance of aged ducks was correlated with follicle decreased numbers and atrophied microstructure, and restricted antioxidant ability of granulosa cells (GCs). Then, according to RNA-Seq analysis, we detected those aged ducks displayed lower Ca concentration in the ovary, while Ca channel related gene expression was increased in GCs to maintain homeostasis. Moreover, the cyclic adenosine monophosphate (cAMP) concentration and cAMP synthase family related genes were also decreased in GCs of aged ducks. Fortunately, medium supplemented with Ca channel-activator A23187 enhanced GC viability, antioxidant ability, tight junction ability, and increased cAMP concentration by improved cAMP metabolism, otherwise, the opposite changes were observed with Ca-chelating agent EGTA or Ca channel-inhibitor Verapamil supplementation. In conclusion, aging disordered Ca signaling to limit cAMP metabolism, then decreased antioxidant ability and inhibited proliferation and migration ability of GCs. Thus, the follicle development and reproductive performance were restricted in aged avian.
卵巢是女性生育能力的主要控制器官，不幸的是，其衰老过程比其他器官更早开始。我们之前的研究表明，在鸟类模型中，钙（Ca）缺乏会降低卵巢重量并减少优势卵泡数量。然而，Ca在老龄鸟类卵泡发育中是否发挥功能作用及其进一步机制仍不清楚。在本研究中，将50只180日龄和50只700日龄的雌性龙岩鸭分为青年组和老龄组，以阐明Ca信号的差异及其进一步机制。我们发现老龄鸭生产性能差与卵泡数量减少、微观结构萎缩以及颗粒细胞（GCs）抗氧化能力受限有关。然后，根据RNA测序分析，我们检测到老龄鸭卵巢中Ca浓度较低，而GCs中与Ca通道相关的基因表达增加以维持体内平衡。此外，老龄鸭GCs中的环磷酸腺苷（cAMP）浓度和与cAMP合酶家族相关的基因也降低。幸运的是，添加Ca通道激活剂A23187的培养基可增强GCs活力、抗氧化能力、紧密连接能力，并通过改善cAMP代谢增加cAMP浓度，否则，添加Ca螯合剂EGTA或Ca通道抑制剂维拉帕米会观察到相反的变化。总之，衰老使Ca信号紊乱，限制了cAMP代谢，进而降低了抗氧化能力，抑制了GCs的增殖和迁移能力。因此，老龄鸟类的卵泡发育和繁殖性能受到限制。
