The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, Shandong, PR China.
Plant Signal Behav. 2021 Jun 3;16(6):1906574. doi: 10.1080/15592324.2021.1906574. Epub 2021 Apr 12.
Metacaspases (MCs), a class of cysteine-dependent proteases, act as important regulators in plant defense response. In maize genome, there are 11 which have been categorized into two types (type I and II) based on their structural differences. In this study, we investigated the different transcript patterns of 11 in maize defense response mediated by the nucleotide-binding, leucine-rich-repeat protein Rp1-D21. We further predicted that many -elements responsive to salicylic acid (SA), methyl jasmonate (MeJA), abscisic acid (ABA) and auxin were identified in the promoter regions of , and several different transcription factors were predicted to bind to their promoters. We analyzed the localization of with previously identified quantitative trait loci (QTLs) in maize disease resistance, and found that all other , except for , are co-located with at least one QTL associated with disease resistance to southern leaf blight, northern leaf blight, gray leaf spot or ear rot. Based on previous RNA-seq analysis, different display different transcript levels in response to and . All the results imply that the members of might have differential functions to different maize diseases. This study lays the basis for further investigating the roles of in maize disease resistance.
类半胱氨酸天冬氨酸蛋白酶（MCs）是一类依赖半胱氨酸的蛋白酶，作为植物防御反应的重要调节剂。在玉米基因组中，有 11 种被分为两类（I 型和 II 型），根据它们的结构差异。在这项研究中，我们研究了核苷酸结合富含亮氨酸重复蛋白 Rp1-D21 介导的玉米防御反应中 11 种的不同转录模式。我们进一步预测，在启动子区域中发现了许多对水杨酸（SA）、茉莉酸甲酯（MeJA）、脱落酸（ABA）和生长素有反应的元件，并且预测了几种不同的转录因子与其启动子结合。我们分析了与玉米抗病性中先前确定的数量性状位点（QTL）的定位，发现除了之外，所有其他的都至少与一个与南方叶斑病、北方叶斑病、灰斑病或穗腐病抗性相关的 QTL 共定位。基于先前的 RNA-seq 分析，不同的在响应和时显示出不同的转录水平。所有的结果都表明，在不同的玉米病害中，类半胱氨酸天冬氨酸蛋白酶的成员可能具有不同的功能。这项研究为进一步研究类半胱氨酸天冬氨酸蛋白酶在玉米抗病性中的作用奠定了基础。
