Satapathy G, Kar S K, Samantaray J C, Panda S K
Division of Microbiology, National Institute of Communicable Diseases, Delhi, India.
Genitourin Med. 1988 Apr;64(2):110-4. doi: 10.1136/sti.64.2.110.
An indirect haemagglutination assay (IHA) with polysaccharide and protein antigens of Trichomonas vaginalis and an enzyme linked immunosorbent assay (ELISA) were used to test for antibodies against T vaginalis in 58 women with trichonomal vaginitis and 48 with non-specific vaginitis. Eleven antibody positive sera were used in a radioimmunoprecipitation assay (RIPA) to identify surface peptides that elicited antibody responses in infected women. The serological tests were less sensitive than biological tests (smear examination and culture); antibodies were detected in 22 of the 58 women with trichomonal vaginitis by IHA using polysaccharide as antigen, in 27 by IHA using protein antigen, and in 36 by ELISA. The ELISA was also found to be of low specificity. Only two of the 11 sera tested by RIPA showed positive reactions with surface antigens, which were confirmed by autoradiography.
采用阴道毛滴虫多糖和蛋白质抗原的间接血凝试验（IHA）以及酶联免疫吸附试验（ELISA），对58例滴虫性阴道炎女性和48例非特异性阴道炎女性进行阴道毛滴虫抗体检测。使用11份抗体阳性血清进行放射免疫沉淀试验（RIPA），以鉴定在感染女性中引发抗体反应的表面肽。血清学检测的敏感性低于生物学检测（涂片检查和培养）；在58例滴虫性阴道炎女性中，以多糖为抗原的IHA检测出22例抗体阳性，以蛋白质抗原的IHA检测出27例，ELISA检测出36例。还发现ELISA的特异性较低。在RIPA检测的11份血清中，只有2份与表面抗原有阳性反应，经放射自显影证实。
