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隼科中一个高度多态性着丝粒串联重复序列的分离与分子特征分析

Isolation and molecular characterization of a highly polymorphic centromeric tandem repeat in the family Falconidae.

作者信息

Longmire J L, Lewis A K, Brown N C, Buckingham J M, Clark L M, Jones M D, Meincke L J, Meyne J, Ratliff R L, Ray F A

机构信息

Life Science Division, Los Alamos National Laboratory, New Mexico 87545.

出版信息

Genomics. 1988 Jan;2(1):14-24. doi: 10.1016/0888-7543(88)90104-8.

DOI:10.1016/0888-7543(88)90104-8
PMID:3384438
Abstract

An abundant tandem repeat has been cloned from genomic DNA of the merlin (Falco columbarius). The cloned sequence is 174 bp in length, and maps by in situ hybridization to the centromeric regions of several of the large chromosomes within the merlin karyotype. Complementary sequences have been identified within a variety of falcon species; these sequences are either absent or in very low copy number in the family Accipitridae. The cloned merlin repeat reveals highly polymorphic restriction patterns in the peregrine falcon (Falco peregrinus). These polymorphisms, which have been shown to be stably inherited within a family of captive peregrines, can be used to differentiate the Greenland and Argentina populations of this endangered raptor species.

摘要

已从矛隼(矛隼)的基因组DNA中克隆出一个丰富的串联重复序列。克隆的序列长度为174 bp,通过原位杂交定位到矛隼核型中几个大染色体的着丝粒区域。在多种隼类物种中已鉴定出互补序列;这些序列在鹰科中要么不存在,要么拷贝数极低。克隆的矛隼重复序列在游隼(矛隼)中显示出高度多态的限制性模式。这些多态性已被证明在圈养游隼家族中可稳定遗传,可用于区分这种濒危猛禽物种的格陵兰和阿根廷种群。

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