De Amaral Faith, Wilson Robert E, Sonsthagen Sarah A, Sehgal Ravinder
San Francisco State University, San Francisco, CA, 94132, United States.
Nebraska State Museum, University of Nebraska-Lincoln, Lincoln, NE, 68588, United States.
Int J Parasitol Parasites Wildl. 2023 Jan 26;20:122-132. doi: 10.1016/j.ijppaw.2023.01.008. eCollection 2023 Apr.
Using samples spanning 10-degrees of latitude in Alaska, we provide the first comparative assessment of avian haemosporidia distribution of Arctic Alaska with subarctic host populations for four species of grouse and three species of ptarmigan (Galliformes). We found a high overall prevalence for at least one haemospordian genus (88%; N = 351/400), with spruce grouse () showing the highest prevalence (100%; N = 54/54). and lineages were only observed within grouse, while species were found within both grouse and ptarmigan. Further, different lineages were obtained from blood and tissue samples from the same individual, potentially due to the differential timing and duration of blood and tissue stages. Using different primer sets, we were able to identify different lineages within 55% (N = 44/80) of sequenced individuals, thereby detecting coinfections that may have otherwise gone undetected. The commonly used / primers amplified for 90% (N = 103/115) of the products sequenced, highlighting the potential value of alternate primers to identify intra-genus coinfections and the importance of obtaining sequence information rather than relying solely on PCR amplification to assess parasite diversity. Overall, this dataset provides baseline information on parasite lineage distributions to assess the range expansion associated with climate change into Arctic regions and underscores methodological considerations for future studies.
利用阿拉斯加跨越10个纬度的样本，我们首次对阿拉斯加北极地区与亚北极地区宿主种群（四种松鸡和三种雷鸟（鸡形目））的鸟类血孢子虫分布进行了比较评估。我们发现至少一种血孢子虫属的总体患病率很高（88%；N = 351/400），其中云杉松鸡（）的患病率最高（100%；N = 54/54）。 和 谱系仅在松鸡中观察到，而 物种在松鸡和雷鸟中均有发现。此外，从同一个体的血液和组织样本中获得了不同的 谱系，这可能是由于血液和组织阶段的时间和持续时间不同。使用不同的引物组，我们能够在55%（N = 44/80）的测序个体中识别出不同的 谱系，从而检测到否则可能未被发现的混合感染。常用的 / 引物对90%（N = 103/115）的测序产物扩增出了 ，突出了替代引物在识别属内混合感染方面的潜在价值以及获取序列信息而非仅依赖PCR扩增来评估寄生虫多样性的重要性。总体而言，该数据集提供了有关寄生虫谱系分布的基线信息，以评估与气候变化相关的范围扩展到北极地区的情况，并强调了未来研究的方法学考虑因素。
