PP7080 通过海绵吸附 miR-670-3p 并调节 UHRF1BP1 促进肺腺癌细胞的增殖和迁移。

PP7080 expedites the proliferation and migration of lung adenocarcinoma cells via sponging miR-670-3p and regulating UHRF1BP1.

机构信息

Department of Oncology, The People's Hospital of Tongcheng, Xianning, Hubei, China.

Cancer Center, People's Hospital of Wuhan University, Wuhan, Hubei, China.

出版信息

J Gene Med. 2021 Aug;23(8):e3341. doi: 10.1002/jgm.3341. Epub 2021 Jun 16.

DOI:10.1002/jgm.3341

PMID:33844396

Abstract

BACKGROUND

An increasing body of evidence has revealed that long non-coding RNAs play a significant part in a variety of human cancers, including lung adenocarcinoma (LUAD).

METHODS

The expression of PP7080, miR-670-3p and UHRF1BP1 in LUAD cells and tissues was detected using a quantitative real-time polymerase chain reaction. The role of PP7080 in LUAD cells was validated by CCK-8, flow cytometry, colony formation, transwell and wound healing assays. The binding capacity between PP7080/UHRF1BP1 and miR-670-3p was confirmed by luciferase reporter assays. Moreover, the interactional mechanism among PP7080, miR-670-3p and UHRF1BP1 was determined by means of RNA immunoprecipitation and western blot assays.

RESULTS

The expression level of PP7080 is up-regulated in LUAD cells and tissues compared to their matched controls. Down-regulation of PP7080 restrained the proliferative and migratory abilities of LUAD cells, but induced cell apoptosis. PP7080 up-regulation led to the opposite results. Moreover, the binding ability between miR-670-3p and PP7080/UHRF1BP1 in LUAD cells was confirmed. A rescue assay revealed that PP7080 contributes to LUAD development by modulating the miR-670-3p/UHRF1BP1 signaling pathway.

CONCLUSIONS

PP7080 expedites the proliferation and migration of LUAD cell via sponging miR-670-3p and modulating UHRF1BP1.

摘要

背景

越来越多的证据表明，长链非编码 RNA 在包括肺腺癌 (LUAD) 在内的多种人类癌症中发挥着重要作用。

方法

采用实时定量聚合酶链反应检测 LUAD 细胞和组织中 PP7080、miR-670-3p 和 UHRF1BP1 的表达。通过 CCK-8、流式细胞术、集落形成、Transwell 和划痕愈合实验验证 PP7080 在 LUAD 细胞中的作用。通过荧光素酶报告基因实验证实 PP7080/UHRF1BP1 与 miR-670-3p 的结合能力。此外，通过 RNA 免疫沉淀和 Western blot 实验确定 PP7080、miR-670-3p 和 UHRF1BP1 之间的互作机制。

结果

与配对对照相比，PP7080 在 LUAD 细胞和组织中的表达水平上调。下调 PP7080 抑制了 LUAD 细胞的增殖和迁移能力，但诱导了细胞凋亡。PP7080 的上调则导致了相反的结果。此外，还证实了 miR-670-3p 与 PP7080/UHRF1BP1 在 LUAD 细胞中的结合能力。挽救实验表明，PP7080 通过调节 miR-670-3p/UHRF1BP1 信号通路促进 LUAD 的发展。