Lamont A G, Bruce M G, Watret K C, Ferguson A
Gastro-Intestinal Unit, University of Edinburgh, Glasgow, U.K.
Immunology. 1988 May;64(1):135-9.
Experiments were designed to examine whether systemic delayed-type hypersensitivity responses (DTH) to ovalbumin (OVA) can be suppressed when antigen is fed after immunization, and to investigate the immunological mechanisms involved. A single 25 mg feed of OVA given 7 or 14 days after immunization with OVA in complete Freund's adjuvant (CFA) suppressed the DTH response of BDF1 mice, but had no significant effect on the serum IgG antibody response. DTH suppression was greatest when antigen was fed soon after immunization, and became less pronounced as the time interval between feeding and immunization increased. The phenomenon was also demonstrated in mice of the BALB/c strain. Cell transfer experiments suggested that the post-immunization suppression was not due to a population of suppressor cells that have been described previously in association with classical oral tolerance for DTH. We conclude that there are separate and distinct mechanisms for the prevention of induction of DTH by antigen feeding in naive mice and the suppression of expression of DTH in sensitized animals.
实验旨在研究在免疫后喂食抗原时，对卵清蛋白（OVA）的全身性迟发型超敏反应（DTH）是否会受到抑制，并探讨其中涉及的免疫机制。在用完全弗氏佐剂（CFA）对OVA进行免疫7天或14天后，单次喂食25 mg OVA可抑制BDF1小鼠的DTH反应，但对血清IgG抗体反应无显著影响。当免疫后不久就喂食抗原时，DTH抑制作用最大，并且随着喂食与免疫之间的时间间隔增加，这种抑制作用变得不那么明显。该现象在BALB/c品系小鼠中也得到了证实。细胞转移实验表明，免疫后的抑制作用并非归因于先前与经典口服DTH耐受性相关的抑制细胞群体。我们得出结论，在未致敏小鼠中通过喂食抗原预防DTH诱导和在致敏动物中抑制DTH表达存在不同且独特的机制。
