Lamont A G, Gordon M, Ferguson A
Immunology. 1987 Jul;61(3):333-7.
We have examined the effects of protein deprivation on the induction of oral tolerance for systemic antibody and DTH responses to the protein antigen ovalbumin (OVA). Mice were fed 4% or 24% protein diets from weaning and given a single feed of OVA 2 weeks later (short-term deprivation) or after 10 weeks (long-term deprivation). Tolerance for serum antibody responses was more profound in protein-deprived animals than in 24% protein-fed control groups. Conversely, tolerance for DTH responses was impaired in protein-deprived mice. This was demonstrated both for short-term deprivation, where nutritional rehabilitation after OVA feeding was necessary to demonstrate this effect on DTH, and for long-term deprivation. Furthermore, the effect of short-term deprivation on tolerance for DTH responses was similar to that observed after cyclophosphamide pretreatment of OVA-fed mice. Protein deprivation has disparate effects on the humoral and cell-mediated limbs of oral tolerance, and our results support the hypothesis that this regime selectively depletes a population of suppressor T cells responsible for the fine control of DTH tolerance.
我们研究了蛋白质缺乏对诱导针对蛋白质抗原卵清蛋白（OVA）的全身抗体和迟发型超敏反应（DTH）的口服耐受的影响。小鼠从断奶开始分别喂食4%或24%蛋白质的饮食，2周后（短期缺乏）或10周后（长期缺乏）给予单次OVA喂食。蛋白质缺乏的动物对血清抗体反应的耐受性比喂食24%蛋白质的对照组更强。相反，蛋白质缺乏的小鼠对DTH反应的耐受性受损。这在短期缺乏中得到了证明，即OVA喂食后需要进行营养恢复才能证明对DTH的这种影响，长期缺乏的情况也是如此。此外，短期缺乏对DTH反应耐受性的影响与OVA喂食小鼠经环磷酰胺预处理后观察到的影响相似。蛋白质缺乏对口服耐受的体液和细胞介导分支有不同影响，我们的结果支持这样的假设，即这种机制选择性地耗尽了负责精细控制DTH耐受性的抑制性T细胞群体。
