Virtanen T, Vilhunen P, Husman K, Happonen P, Mäntyjärvi R
Department of Clinical Microbiology, University of Kuopio, Finland.
Int Arch Occup Environ Health. 1988;60(5):355-60. doi: 10.1007/BF00405670.
Samples of airborne particulate material were collected from 18 Finnish cowsheds both at stationary sites and in breathing zones. The concentrations of bovine epithelial antigen (BEA) in samples were analyzed by the immunochemical enzyme-linked immunosorbent assay (ELISA) inhibition test. For total dust and BEA measurements the range of results among cowsheds was wide. In the breathing zone the mean concentration of total dust was 2.4 +/- 1.5 mg/m3 and of BEA 460 +/- 300 ng/m3. The concentrations of total dust and BEA were significantly lower at stationary sites than in breathing zones. Correlation analyses of total dust and BEA concentrations revealed that only moderate correlations existed, and they were not statistically significant (P greater than 0.05). Correlations between BEA concentrations in feeding passage or manure passage and breathing zone were at most only moderate (rs 0.29, P = 0.12 and rs 0.42, P = 0.02, respectively). The correlation of BEA concentrations between the two stationary sites was relatively strong and significant (rs 0.71, P = 0.004), suggesting that the variation in BEA concentrations between cowsheds is real. The effects of brushing and number of animals on total dust and BEA concentrations were studied statistically in samples from the breathing zone. No high or significant correlations were observed.
从芬兰的18个奶牛棚的固定地点和呼吸区域采集了空气传播颗粒物样本。通过免疫化学酶联免疫吸附测定（ELISA）抑制试验分析样本中牛上皮抗原（BEA）的浓度。奶牛棚之间总粉尘和BEA测量结果的范围很广。在呼吸区域，总粉尘的平均浓度为2.4±1.5毫克/立方米，BEA为460±300纳克/立方米。固定地点的总粉尘和BEA浓度明显低于呼吸区域。总粉尘和BEA浓度的相关性分析表明，仅存在中等相关性，且无统计学意义（P>0.05）。喂食通道或粪便通道中的BEA浓度与呼吸区域之间的相关性至多为中等（rs分别为0.29，P = 0.12和rs 0.42，P = 0.02）。两个固定地点之间BEA浓度的相关性相对较强且具有统计学意义（rs 0.71，P = 0.004），这表明奶牛棚之间BEA浓度的差异是真实存在的。对呼吸区域样本中刷拭和动物数量对总粉尘和BEA浓度的影响进行了统计研究。未观察到高度或显著的相关性。
