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Estimation of airborne rat-derived antigens by ELISA.

作者信息

Davies G E, Thompson A V, Rackham M

出版信息

J Immunoassay. 1983;4(2):113-26. doi: 10.1080/15321818308057004.

DOI:10.1080/15321818308057004
PMID:6350369
Abstract

Double antibody sandwich ELISA procedures have been devised to estimate the concentrations of airborne rat urinary and epidermal antigens. Both assays were capable of detecting at least 3 ng/ml of antigen protein. Unknown concentrations were estimated in terms of the standard, laboratory-prepared material, as calculated from the linear relationship between absorbance and log concentration. The coefficient of variation of a single assay was 12%, the methods have been applied to extracts of the filter material from static and personal samplers in an animal room.

摘要

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