Twiggs J T, Agarwal M K, Dahlberg M J, Yunginger J W
J Allergy Clin Immunol. 1982 Jun;69(6):522-6. doi: 10.1016/0091-6749(82)90177-4.
The major allergens from the mouse, including mouse serum albumin (MSA) and mouse urinary protein complex, are present in various concentrations in urine, serum, and pelts of mice. Subjects clinically sensitive to laboratory animals were screened by the RAST for specific IgE antibodies to mouse urine (MU), MSA, and mouse pelt extract (MPE). Twenty-four hour air samples from both a mouse-care room (2000 mice) and an immunology laboratory (five to 100 mice) were collected with a high-volume air sampler that retained particles greater than 0.3 micron with 95% efficiency. Eluates from the filter sheets were dialyzed, lyophilized, reconstituted in water, and used as fluid-phase inhibitors in the MU RAST and MPE RAST. MPE allergenic activity could be quantitated in filter eluates from both locations, but MU allergenic activity could be quantitated only in the mouse-care room. Airborne allergen content ranged from 1.8 to 825 ng/m3 and varied with both the number of mice and the degree of work activity in the rooms. The sampling and assay techniques described can be used to accurately quantitate amorphous airborne allergens.
来自小鼠的主要过敏原，包括小鼠血清白蛋白（MSA）和小鼠尿蛋白复合物，在小鼠的尿液、血清和皮毛中以不同浓度存在。通过放射变应原吸附试验（RAST）对临床上对实验动物敏感的受试者进行筛查，以检测其针对小鼠尿液（MU）、MSA和小鼠皮毛提取物（MPE）的特异性IgE抗体。使用大容量空气采样器收集来自一个饲养2000只小鼠的动物饲养室和一个免疫实验室（5至100只小鼠）的24小时空气样本，该采样器能以95%的效率截留大于0.3微米的颗粒。将滤纸片的洗脱液进行透析、冻干、用水复溶，并用作MU RAST和MPE RAST中的液相抑制剂。在两个地点的滤纸片洗脱液中都可以定量MPE的变应原活性，但只有在动物饲养室中才能定量MU的变应原活性。空气中变应原含量范围为1.8至825 ng/m³，且随室内小鼠数量和工作活动程度而变化。所描述的采样和检测技术可用于准确定量无定形空气传播变应原。
