National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China.
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China; School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, China.
Environ Res. 2021 Jun;197:111119. doi: 10.1016/j.envres.2021.111119. Epub 2021 Apr 9.
The microorganisms in marine sediment are promising candidates for the treatment of the saline wastes due to their property of salt tolerance. However, the knowledge about the microbial community and property of the marine sediments is still limited. In the present study, the salt tolerance of the microorganisms in the marine sediment that was collected from a marine fish farm was investigated by being used as inoculum for anaerobic digestion. The microbial communities were analyzed by high-throughput sequencing. The inoculum from the wastewater plant (IWTP) was taken as a control. The inoculum from the marine sediment (IMS) showed excellent capacity for anaerobic digestion at salinities of 0.3%-6%. Even at a salinity of 9%, the methane yield remained 60% of the highest yield. IMS provides promising microbial resources for the treatment of both fresh-water and saliferous organic wastes. While the IWTP was sensitive to salt, the methane yield decreased to 56% of the highest yield at the salinity of 3%. The bacterial taxonomic richness of IMS was about half of that in IWTP. Eighty-one genera were identified only in IWTP but not in IMS. The IMS possessed fewer bacterial members related to the nitrogen cycle than IWTP, but more members related to the sulfur cycle. The members of animal parasites or symbionts in IMS were significantly fewer than those in IWTP. The archaeal compositions of IMS and IWTP were different. The relative abundance of the unidentified archaea in IMS was much higher than that in IWTP with 12.52% vs 0.06% at phylum level. The findings of this work expand our understanding of the microorganisms in marine sediments and will promote the application of them in waste treatment.
海洋沉积物中的微生物由于其耐盐性而成为处理含盐废物的有前途的候选物。然而，关于海洋沉积物中的微生物群落和性质的知识仍然有限。在本研究中，使用从海洋养殖场收集的海洋沉积物中的微生物作为接种物来进行厌氧消化，从而研究了海洋沉积物中微生物的耐盐性。通过高通量测序分析微生物群落。以废水处理厂（IWTP）的接种物作为对照。来自海洋沉积物（IMS）的接种物在盐度为 0.3%-6%的条件下表现出出色的厌氧消化能力。即使在盐度为 9%的条件下，甲烷产率仍保持最高产率的 60%。IMS 为处理淡水和含盐有机废物提供了有前途的微生物资源。而 IWTP 对盐敏感，当盐度为 3%时，甲烷产率下降到最高产率的 56%。IMS 的细菌分类丰富度约为 IWTP 的一半。只有在 IWTP 中鉴定出 81 个属，而在 IMS 中则没有。IMS 中与氮循环有关的细菌成员比 IWTP 少，但与硫循环有关的成员更多。IMS 中的动物寄生虫或共生体成员明显少于 IWTP。IMS 和 IWTP 的古菌组成不同。IMS 中未鉴定古菌的相对丰度比 IWTP 高得多，在门水平上分别为 12.52%和 0.06%。这项工作的发现扩展了我们对海洋沉积物中微生物的理解，并将促进它们在废物处理中的应用。
