State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
Jiaxing Zeyuan Bio-Products Limited Company, Jiaxing 314006, PR China.
Bioresour Technol. 2015 Dec;198:557-63. doi: 10.1016/j.biortech.2015.09.031. Epub 2015 Sep 18.
A novel cultivation strategy called "Sequential Heterotrophy-Dilution-Photoinduction" was successfully applied in the cultivation of Haematococcus pluvialis to produce astaxanthin effectively. Cells were first cultivated heterotrophically to achieve a high cell density, then were diluted to a suitable concentration and switched to a favorable environment for cells acclimation. Finally, the culture was transferred to high light environment for astaxanthin accumulation. By this strategy, the dry cell weight of 26 g/L and biomass productivity of 64.1mg/L/h were obtained in heterotrophy stage which surpassed ever before reported in literatures. Meanwhile, the cells could accumulate considerable astaxanthin up to 4.6% of dry cell weight after 10 days of photoinduction. Furthermore, the application prospects of the strategy were confirmed further by outdoor experiments. Therefore, this novel strategy provided a promising approach for high-efficient production of natural astaxanthin from H. pluvialis to meet the huge demand of this high value product.
一种名为“顺序异养-稀释-光诱导”的新型培养策略被成功应用于雨生红球藻的培养，有效地生产虾青素。细胞首先进行异养培养以达到高细胞密度，然后稀释到合适的浓度，并切换到有利于细胞适应的环境。最后，将培养物转移到高光环境中进行虾青素积累。通过该策略，在异养阶段获得了 26g/L 的干细胞重量和 64.1mg/L/h 的生物量生产率，超过了文献中以往的报道。同时，经过 10 天的光诱导，细胞可以积累相当可观的虾青素，达到干细胞重量的 4.6%。此外，该策略的应用前景通过户外实验得到了进一步证实。因此，这种新型策略为从雨生红球藻高效生产天然虾青素提供了一种有前途的方法，以满足这种高价值产品的巨大需求。
