Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Anticancer Agents Med Chem. 2022;22(7):1386-1396. doi: 10.2174/1871520621666210412113944.
Triple-negative breast cancer (TNBC) accounts for 15% of all breast cancer (BC) cases and is a severe type of BC. Since medicinal herbs containing biocompatible substances that are accepted by patient more than chemical therapeutics, they can be considered a safe option for treating BC.
This study evaluated the effect of Sambucus Ebulus (S. ebulus) extract on a model of TNBC.
S. ebulus extract was prepared using petroleum ether, ethyl acetate, and methanol. The petroleum ether extract was fractionated and analyzed using vacuum liquid chromatography and GC-MS, respectively. MDAMB- 231 and MCF-10A were used as TNBC and normal breast cells, respectively. Flowcytometry and MTT assays were performed to evaluate cell cycle, apoptosis, and viability of the cells. Gene expression analysis was performed using RT-qPCR. Nude mouse allograft tumor models were used, and pathological sections were evaluated.
The findings indicated that S. ebulus extract remarkably decreased cell proliferation and viability. The extract had no toxicity to the normal breast cells but efficiently killed the cancer cells. Cell cycle- and apoptosisrelated gene expression showed that fraction 4 of S. ebulus extract significantly increased the expression of Bax, Bak, P53, and c-MYC.
This study showed satisfactory results of the effect of S. ebulus extract on clearing BC cells both in vitro and in vivo. Thus, S. ebulus extract may be a safe herbal compound for eliminating BC cells without toxicity to host cells.
三阴性乳腺癌（TNBC）占所有乳腺癌（BC）病例的 15%，是一种严重的 BC 类型。由于草药含有比化学疗法更能被患者接受的生物相容性物质，因此它们可以被认为是治疗 BC 的安全选择。
本研究评估了接骨木（Sambucus ebulus）提取物对 TNBC 模型的影响。
使用石油醚、乙酸乙酯和甲醇制备接骨木提取物。分别使用真空液相色谱和 GC-MS 对石油醚提取物进行馏分分析和分析。MDAMB-231 和 MCF-10A 分别用作 TNBC 和正常乳腺细胞。通过流式细胞术和 MTT 分析评估细胞周期、细胞凋亡和细胞活力。使用 RT-qPCR 进行基因表达分析。使用裸鼠异种移植肿瘤模型，并评估病理切片。
研究结果表明，接骨木提取物显著降低了细胞增殖和活力。该提取物对正常乳腺细胞没有毒性，但能有效杀死癌细胞。细胞周期和凋亡相关基因表达表明，接骨木提取物的第 4 馏分显着增加了 Bax、Bak、P53 和 c-MYC 的表达。
本研究表明接骨木提取物在体外和体内清除 BC 细胞的效果令人满意。因此，接骨木提取物可能是一种安全的草药化合物，可消除 BC 细胞而不对宿主细胞产生毒性。
