Ghaffari Hadi, Ataei-Pirkooh Angila, Mirghazanfari Sayid Mahdi, Barati Mohammad
Infectious Diseases Research Center, AJA University of Medical Sciences, Tehran, Iran.
Department of Bacteriology and Virology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Med J Islam Repub Iran. 2021 Jan 18;35:9. doi: 10.47176/mjiri.35.9. eCollection 2021.
The emergence of drug-resistant strains of herpes simplex virus type 1 (HSV-1) has been increasingly reported. Therefore, attempts to discover new antiviral agents in particular from natural compounds are required. In this study, we evaluated the possible inhibitory effects of hydroalcoholic extract of ( ) against HSV-1. extract was produced by maceration method. MTT assay was used to evaluate the cytotoxicity effects of the extract; also, antiviral effects were measured both by test TCID50 and quantitative real-time PCR methods. To study the inhibitory impact of S. ebulus extract on the expression of HSV-1 antigens, indirect immunofluorescence assay (IFA) was also performed. All analyses were performed using the GraphPad Prism software v. 7.0. In the postexposure assay of HSV-1 with extract at the highest nontoxic concentration (75 μg/mL), extract led to 2.6 log10 TCID50 reduction in infectious virus titer. At the highest nontoxic concentration, the extract led to inhibition rates of 91.2%, based on the quantitative real-time PCR assay results (p<0.001). Also, in the immunofluorescence assay, a significant reduction was observed in fluorescence emission intensity in HSV-1-infected cell treated with extract compared to the control group. extract is a novel and effective natural compound in reducing HSV-1 titer and future studies should be conducted to discover the complete mechanism of antiviral effect of this natural compound.
1型单纯疱疹病毒（HSV-1）耐药菌株的出现报道日益增多。因此，需要尝试从天然化合物中发现新的抗病毒药物。在本研究中，我们评估了（ ）水醇提取物对HSV-1的可能抑制作用。提取物通过浸渍法制备。采用MTT法评估提取物的细胞毒性作用；同时，通过检测TCID50和定量实时PCR方法测定抗病毒作用。为研究欧夏至草提取物对HSV-1抗原表达的抑制作用，还进行了间接免疫荧光试验（IFA）。所有分析均使用GraphPad Prism软件v. 7.0进行。在以最高无毒浓度（75μg/mL）的提取物对HSV-1进行暴露后试验中，提取物使感染性病毒滴度降低了2.6 log10 TCID50。根据定量实时PCR检测结果，在最高无毒浓度下，提取物的抑制率为91.2%（p<0.001）。此外，在免疫荧光试验中，与对照组相比，用提取物处理的HSV-1感染细胞的荧光发射强度显著降低。提取物是一种降低HSV-1滴度的新型有效天然化合物，未来应开展研究以发现这种天然化合物抗病毒作用的完整机制。
