评价氧化镁和氧化锌纳米颗粒对多药耐药结核分枝杆菌的作用。
Evaluation of magnesium oxide and zinc oxide nanoparticles against multi-drug-resistance Mycobacterium tuberculosis.
机构信息
Department of Health, Nutrition & Infectious Diseases, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Inflammatory Lung Disease Research Center, Department of Internal Medicine, Razi Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran; Urology Research Center, Razi Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
出版信息
Indian J Tuberc. 2021 Apr;68(2):195-200. doi: 10.1016/j.ijtb.2020.07.032. Epub 2020 Aug 4.
OBJECTIVE
The current study has evaluated the MICs and MBCs of ZnONPs, MgONPs, and MgONPs-ZnONPs against HRv Mtb and MDR-Mtb.
METHODS
Mixture, magnesium oxide nanoparticles (NPs) and zinc oxide (MgONPs-ZnONPs) were prepared. The microplate alamar blue (MABA) assay and the proportion method were used to evaluate of anti-tubercular activity against MDR-MTB. MTT test was done to MgONPs-ZnONPs against Vero and HepG cell lines.
RESULTS
The MIC of MgONPs and ZnONPs were 0.195 and 0.468 μg mL against 10 of HRv Mtb. As well, 0.166 μg mL of MgONPs-ZnONPs was able to inhibit 10 HRv Mtb. The MIC of MgONPs against 10 concentrations of MDR-Mtb was 12.5 μg mL. The MIC of MgONPs/ZnONPs against 10 concentrations of MDR-Mtb reached to 0.664 μg mL. The MBC value of ZnONPs increased to 1.875 μg mL against 10 concentrations of MDR-Mtb. Testing showed that the MBCs of MgONPs/ZnONPs reached to 1.328 μg mL against 10 concentrations of MDR-Mtb. The IC against MDR-TB was 0.779 μg mL for ZnONPs and 0.883 μg mL for MgONPs-ZnONPs. The MgONPs-ZnONPs was not toxic to Vero cell lines however ZnONPs could inhibit the Vero and HepG cell lines.
CONCLUSION
We found that ZnONPs and mixture MgONPs-ZnONPs not only have higher bactericide behavior but might have also synergistic effects against MDR-TB.
目的
本研究评估了 ZnONPs、MgONPs 和 MgONPs-ZnONPs 对 HRv Mtb 和 MDR-Mtb 的 MIC 和 MBC。
方法
制备混合物、氧化镁纳米颗粒 (NPs) 和氧化锌 (MgONPs-ZnONPs)。使用微孔板溴甲酚紫 (MABA) 测定法和比例法评估 MDR-MTB 的抗结核活性。MTT 试验用于评估 MgONPs-ZnONPs 对 Vero 和 HepG 细胞系的作用。
结果
MgONPs 和 ZnONPs 对 10 株 HRv Mtb 的 MIC 分别为 0.195 和 0.468 μg/mL。同样，0.166 μg/mL 的 MgONPs-ZnONPs 能够抑制 10 株 HRv Mtb。MgONPs 对 10 浓度 MDR-Mtb 的 MIC 为 12.5 μg/mL。MgONPs/ZnONPs 对 10 浓度 MDR-Mtb 的 MIC 达到 0.664 μg/mL。ZnONPs 对 10 浓度 MDR-Mtb 的 MBC 值增加到 1.875 μg/mL。试验表明，MgONPs/ZnONPs 对 10 浓度 MDR-Mtb 的 MBC 值达到 1.328 μg/mL。ZnONPs 对 MDR-TB 的 IC 为 0.779 μg/mL，MgONPs-ZnONPs 为 0.883 μg/mL。MgONPs-ZnONPs 对 Vero 细胞系没有毒性，但 ZnONPs 可以抑制 Vero 和 HepG 细胞系。
结论
我们发现 ZnONPs 和混合 MgONPs-ZnONPs 不仅具有更高的杀菌行为，而且可能对 MDR-TB 具有协同作用。
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